A HIGH-MOLECULAR-WEIGHT PROTEOGLYCAN IS DIFFERENTIALLY EXPRESSED DURING DEVELOPMENT OF THE MOLLUSK CONCHOLEPAS-CONCHOLEPAS (MOLLUSCA, GASTROPODA, MURICIDAE)

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dc.contributor.authorBRANDAN, E
dc.contributor.authorGONZALEZ, M
dc.contributor.authorINESTROSA, NC
dc.contributor.authorTREMBLAY, C
dc.contributor.authorURREA, R
dc.date.accessioned2025-01-23T19:21:58Z
dc.date.available2025-01-23T19:21:58Z
dc.date.issued1992
dc.description.abstractIncorporation of radioactive sulfate to hatched veliger larvae of the gastropod muricid Concholepas concholepas indicated that over 87% of the sulfated macromolecules were found in the detergent insoluble fraction, rich in extracellular matrix (ECM) components. The sulfated material was solubilized with guanidine salt followed by urea dialysis and fractionated by DEAE-Sephacel chromatography. Three sulfated compounds eluting at 0.7, 1.1, and 3.0 M NaCl, called peaks I, II, and III, respectively, were obtained. The sulfated compound present in peak I was degraded by pronase or sodium alkaline treatment to a small sulfated resistant material, suggesting the presence of a proteoglycan (PG). Filtration analysis on Sephacryl S-500 and SDS-PAGE of the intact PG indicates that it has a high molecular weight (360,000 to over 1 X 10(6)). Monoclonal antibodies (mAb) against this PG were produced. The specificity of one mAb, the 6H2, was demonstrated by size chromatography and ELISA analysis. The epitope recognized by this mAb seems to be present in the core protein of the PG. Both the extent of sulfation and the presence of different sulfated species of PGs were evaluated during the development of this mollusc. A twelvefold increase in the incorporation of sulfate to PGs per milligram of protein was found in veliger larvae compared to blastula-glastula stages. This change correlated well with the differential expression of the sulfated PG present in peak I. Biochemical and immunological analysis indicate that high levels of this PG are found in veliger and trocophore larvae in comparison with blastula-gastrula and early juveniles. These results indicate that a high molecular weight PG probably of the ECM is differentially expressed during the development of the gastropod Concholepas concholepas.
dc.fuente.origenWOS
dc.identifier.issn0022-104X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98921
dc.identifier.wosidWOS:A1992KD20500001
dc.issue.numero4
dc.language.isoen
dc.pagina.final371
dc.pagina.inicio363
dc.revistaJournal of experimental zoology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA HIGH-MOLECULAR-WEIGHT PROTEOGLYCAN IS DIFFERENTIALLY EXPRESSED DURING DEVELOPMENT OF THE MOLLUSK CONCHOLEPAS-CONCHOLEPAS (MOLLUSCA, GASTROPODA, MURICIDAE)
dc.typeartículo
dc.volumen264
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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