Identification of genes expressed during early somatic embryogenesis in <i>Pinus radiata</i>

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dc.contributor.authorAquea, Felipe
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-21T01:05:06Z
dc.date.available2025-01-21T01:05:06Z
dc.date.issued2008
dc.description.abstractAnalysis of cDNA-AFLPs was used to study gene expression underlying the early embryogenic process in the gymnosperm Pinus radiata. Somatic embryogenesis in this species was used as a model as it resulted in the generation of a large number of embryos at defined stages of development. The gene expression patterns of three ernbryogenic stages were compared with non-embryogenic cells. Fifty transcript-derived fragments (TDFs) that are upregulated and 32 TDFs that are down-regulated in the embryogenic stages were selected, sequenced and their homologies sought in the databases. Expression of a selected subset of differentially expressed genes was confirmed by RT-PCR and their levels of expression were quantified. Of the 50 up-regulated TDFs, 16 are homologous to genes encoding either known or putative proteins in higher plants, 19 are homologous to conifer ESTs and 15 did not show significant matches. Of the down-regulated TDFs, 8 are homologous to genes encoding either known or putative proteins, 20 are homologous to conifer ESTs and 4 of them did not show significant matches in DNA or protein sequence database. The known up-regulated genes were similar to genes involved in cellular metabolism and in the stress response and the known down-regulated genes were similar to genes involved in proteolysis, cell wall modification and signaling pathways. Their putative individual function is briefly reviewed based on published information, and the potential roles of these genes in embryo development are discussed. (C) 2008 Elsevier Masson SAS. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.plaphy.2008.02.012
dc.identifier.eissn1873-2690
dc.identifier.issn0981-9428
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2008.02.012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95817
dc.identifier.wosidWOS:000257376500005
dc.issue.numero5-6
dc.language.isoen
dc.pagina.final568
dc.pagina.inicio559
dc.revistaPlant physiology and biochemistry
dc.rightsacceso restringido
dc.subjectcDNA-AFLP
dc.subjectplant embryogenesis
dc.subjectpine genomics
dc.subjectPinus radiata
dc.subjectconifer
dc.subjectgene expression
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleIdentification of genes expressed during early somatic embryogenesis in <i>Pinus radiata</i>
dc.typeartículo
dc.volumen46
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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