<i>Neurod4</i> converts endogenous neural stem cells to neurons with synaptic formation after spinal cord injury

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dc.contributor.authorFukuoka, Toshiki
dc.contributor.authorKato, Akira
dc.contributor.authorHirano, Masaki
dc.contributor.authorOhka, Fumiharu
dc.contributor.authorAoki, Kosuke
dc.contributor.authorAwaya, Takayuki
dc.contributor.authorAdilijiang, Alimu
dc.contributor.authorSachi, Maeda
dc.contributor.authorTanahashi, Kuniaki
dc.contributor.authorYamaguchi, Junya
dc.contributor.authorMotomura, Kazuya
dc.contributor.authorShimizu, Hiroyuki
dc.contributor.authorNagashima, Yoshitaka
dc.contributor.authorAndo, Ryo
dc.contributor.authorWakabayashi, Toshihiko
dc.contributor.authorLee-Liu, Dasfne
dc.contributor.authorLarrain, Juan
dc.contributor.authorNishimura, Yusuke
dc.contributor.authorNatsume, Atsushi
dc.date.accessioned2025-01-20T23:52:23Z
dc.date.available2025-01-20T23:52:23Z
dc.date.issued2021
dc.description.abstractThe transcriptome analysis of injured Xenopus laevis tadpole and mice suggested that Neurod4L.S., a basic-helix-loop-helix transcription factor, was the most promising transcription factor to exert neuroregeneration after spinal cord injury (SCI) in mammals. We generated a pseudotyped retroviral vector with the neurotropic lymphocytic choriomeningitis virus (LCMV) envelope to deliver murine Neurod4 to mice undergoing SCI. SCI induced ependymal cells to neural stem cells (NSCs) in the central canal. The LCMV envelope-based pseudotypedvector preferentially introduced Neurod4 into activated NSCs, which converted to neurons with axonal regrowth and suppressed the scar-forming glial lineage. Neurod4-induced inhibitory neurons predominantly projected to the subsynaptic domains of motor neurons at the epicenter, and Neurod4-induced excitatory neurons predominantly projected to subsynaptic domains of motor neurons caudal to the injury site suggesting the formation of functional synapses. Thus, Neurod4 is a potential therapeutic factor that can improve anatomical and functional recovery after SCI.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.isci.2021.102074
dc.identifier.eissn2589-0042
dc.identifier.urihttps://doi.org/10.1016/j.isci.2021.102074
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94909
dc.identifier.wosidWOS:000621266700049
dc.issue.numero2
dc.language.isoen
dc.revistaIscience
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.title<i>Neurod4</i> converts endogenous neural stem cells to neurons with synaptic formation after spinal cord injury
dc.typeartículo
dc.volumen24
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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