Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in <i>Nicotiana tabacum</i> plants

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dc.contributor.authorStange, C
dc.contributor.authorMatus, JT
dc.contributor.authorElorza, A
dc.contributor.authorArce-Johnson, P
dc.date.accessioned2025-01-21T01:08:31Z
dc.date.available2025-01-21T01:08:31Z
dc.date.issued2004
dc.description.abstractNicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs ( bp) long and has 82.6% nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR/NSB/LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants ( N. tabacum Xanthi NN,N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene ( GenBank acc. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.
dc.fuente.origenWOS
dc.identifier.doi10.1071/FP03160
dc.identifier.eissn1445-4416
dc.identifier.issn1445-4408
dc.identifier.urihttps://doi.org/10.1071/FP03160
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96477
dc.identifier.wosidWOS:000220001900006
dc.issue.numero2
dc.language.isoen
dc.pagina.final158
dc.pagina.inicio149
dc.revistaFunctional plant biology
dc.rightsacceso restringido
dc.subjectdisease resistance
dc.subjectHR-like
dc.subjecthypersensitive response
dc.subjectN gene
dc.subjectTIR/NBS/LRR receptor
dc.subjectTMV-Cg
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleIdentification and characterization of a novel tobacco mosaic virus resistance N gene homologue in <i>Nicotiana tabacum</i> plants
dc.typeartículo
dc.volumen31
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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