Spatiotemporal analysis identifies ABF2 and ABF3 as key hubs of endodermal response to nitrate

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dc.contributor.authorContreras-Lopez, Orlando
dc.contributor.authorVidal, Elena A.
dc.contributor.authorRiveras, Eleodoro
dc.contributor.authorAlvarez, Jose M.
dc.contributor.authorMoyano, Tomas C.
dc.contributor.authorSparks, Erin E.
dc.contributor.authorMedina, Joaquin
dc.contributor.authorPasquino, Angelo
dc.contributor.authorBenfey, Philip N.
dc.contributor.authorCoruzzi, Gloria M.
dc.contributor.authorGutierrez, Rodrigo A.
dc.date.accessioned2025-01-20T21:12:22Z
dc.date.available2025-01-20T21:12:22Z
dc.date.issued2022
dc.description.abstractNitrate is a nutrient and a potent signal that impacts global gene expression in plants. However, the regulatory factors controlling temporal and cell type-specific nitrate responses remain largely unknown. We assayed nitrate-responsive transcriptome changes in five major root cell types of the Arabidopsis thaliana root as a function of time. We found that gene-expression response to nitrate is dynamic and highly localized and predicted cell type- specific transcription factor (TF)-target interactions. Among cell types, the endodermis stands out as having the largest and most connected nitrate-regulatory gene network. ABF2 and ABF3 are major hubs for transcriptional responses in the endodermis cell layer. We experimentally validated TF-target interactions for ABF2 and ABF3 by chromatin immunoprecipitation followed by sequenc-ing and a cell-based system to detect TF regulation genome-wide. Validated targets of ABF2 and ABF3 account for more than 50% of the nitrate-responsive transcriptome in the endodermis. Moreover, ABF2 and ABF3 are involved in nitrate-induced lateral root growth. Our approach offers an unprecedented spatiotemporal resolution of the root response to nitrate and identifies important compo-nents of cell-specific gene regulatory networks.
dc.fuente.origenWOS
dc.identifier.doi10.1073/pnas.2107879119
dc.identifier.eissn1091-6490
dc.identifier.issn0027-8424
dc.identifier.urihttps://doi.org/10.1073/pnas.2107879119
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93656
dc.identifier.wosidWOS:000758466500019
dc.issue.numero4
dc.language.isoen
dc.revistaProceedings of the national academy of sciences of the united states of america
dc.rightsacceso restringido
dc.subjectcell-specific
dc.subjectnitrate signaling
dc.subjecttranscriptional regulation
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleSpatiotemporal analysis identifies ABF2 and ABF3 as key hubs of endodermal response to nitrate
dc.typeartículo
dc.volumen119
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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