CALMODULIN BINDS TO A TUBULIN BINDING-SITE OF THE MICROTUBULE-ASSOCIATED PROTEIN-TAU

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dc.contributor.authorPADILLA, R
dc.contributor.authorMACCIONI, RB
dc.contributor.authorAVILA, J
dc.date.accessioned2025-01-23T19:23:04Z
dc.date.available2025-01-23T19:23:04Z
dc.date.issued1990
dc.description.abstractPrevious studies have demonstrated that the microtubule-associated proteins MAP-2 and tau interact selectively with common binding domains on tubulin defined by the low-homology segments .alpha.(430-441) and .beta. (422-434). It has been also indicated that the synthetic peptide VRSKIGSTENLKHQPGGG corresponding to the first tau repetitive sequence represents a tubulin binding domain on tau. The present studies show that the calcium-binding protein calmodulin interacts with a tubulin binding site on tau defined by the second repetitive sequence VTSKCGSLGNIHHKPGGG. It was shown that both tubulin and calmodulin bind to tau peptide-Sepharose affinity column. Binding of calmodulin occurs in the presence of 1 mM Ca2 + and it can be eluted from the column with 4 mM EGTA. These findings provide new insights into the regulation of microtubule assembly, since Ca2 +/calmodulin inhibition of tubulin polymerization into microtubules could be mediated by the direct binding of calmodulin to tau, thus preventing the interaction of this latter protein with tubulin.
dc.fuente.origenWOS
dc.identifier.eissn1573-4919
dc.identifier.issn0300-8177
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99156
dc.identifier.wosidWOS:A1990DW30900004
dc.issue.numero1
dc.language.isoen
dc.pagina.final41
dc.pagina.inicio35
dc.revistaMolecular and cellular biochemistry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCALMODULIN BINDS TO A TUBULIN BINDING-SITE OF THE MICROTUBULE-ASSOCIATED PROTEIN-TAU
dc.typeartículo
dc.volumen97
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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