Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen <i>Piscirickettsia salmonis</i>

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dc.contributor.authorWilhelm, V
dc.contributor.authorMorales, C
dc.contributor.authorMartínez, R
dc.contributor.authorRosemblatt, M
dc.contributor.authorBurzio, LO
dc.contributor.authorValenzuela, PDT
dc.date.accessioned2025-01-21T01:08:23Z
dc.date.available2025-01-21T01:08:23Z
dc.date.issued2004
dc.description.abstractWe have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong Immoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant proteins. A similar response was elicited by both proteins when injected intraperitoneally in Atlantic salmon. The present data indicates that these proteins are good candidates to be used in formulations to study the protective immunity of salmon to infection by P. salmonis.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96461
dc.identifier.wosidWOS:000225458100007
dc.issue.numero4
dc.language.isoen
dc.pagina.final793
dc.pagina.inicio783
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectPiscirickettsia salmonis
dc.subjectMltB
dc.subjectTbpB
dc.subjectrecombinant antigens
dc.subjectrecombinant vaccines
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIsolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen <i>Piscirickettsia salmonis</i>
dc.typeartículo
dc.volumen37
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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