Antigen retrieval by citrate solution improves western blot signal

Simple item page
dc.contributor.authorPatino-Garcia, Daniel
dc.contributor.authorRocha-Perez, Nadia
dc.contributor.authorMoreno, Ricardo D.
dc.contributor.authorOrellana, Renan
dc.date.accessioned2025-01-23T21:10:01Z
dc.date.available2025-01-23T21:10:01Z
dc.date.issued2019
dc.description.abstractIn the present work, we describe and evaluate an additional step to the standard western blot protocol to increase signal strength after revealing. Weak or absence of signal is a common issue in western blot protocol leading to unexpected results. In our Antigen Retrieval for Western Blot Method (ARWB method), after transfer, the membrane was incubated in a citrate buffer following normal antigen retrieval procedure used for immunohistochemistry. Later, standard protocol was performed in order to reveal and compare with unexposed membranes to this antigen retrieval step. Signal in bands obtained by the modified protocol resulted significantly higher (in all 13 antibodies analyzed) compared to standard protocol. Some bands were only visible after citrate incubation. This method is a simple and economical way to improve results in western blot analysis.
dc.description.abstractThe ARWB method significantly increases band's density in all antibodies analyzed.
dc.description.abstractProtein localization does not influence the efficacy of the ARWB method since membrane and citoplasmatic proteins bands increase their signal in a similar way after the protocol is performed.
dc.description.abstractThis ARWB method is simple, safe, economical and undoubtedly helpful in immunoblotting for proteins with weak signal. (C) 2019 The Authors. Published by Elsevier B.V.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.mex.2019.02.030
dc.identifier.eissn2215-0161
dc.identifier.urihttps://doi.org/10.1016/j.mex.2019.02.030
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100832
dc.identifier.wosidWOS:000493729600054
dc.language.isoen
dc.pagina.final468
dc.pagina.inicio464
dc.revistaMethodsx
dc.rightsacceso restringido
dc.subjectAntigen retrieval
dc.subjectSDS-PAGE
dc.subjectCitrate buffer
dc.subjectAntibody/epitope interaction
dc.subjectNovel step
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAntigen retrieval by citrate solution improves western blot signal
dc.typeartículo
dc.volumen6
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas