SPECIFIC BINDING OF L-[H-3]-GLUTAMIC ACID TO RAT SUBSTANTIA-NIGRA SYNAPTIC-MEMBRANES

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dc.contributor.authorFIEDLER, JL
dc.contributor.authorARQUEROS, L
dc.contributor.authorBUSTOS, G
dc.date.accessioned2025-01-23T19:26:39Z
dc.date.available2025-01-23T19:26:39Z
dc.date.issued1986
dc.description.abstractThe specific binding of L-[3H]-glutamic acid (GLU) was investigated in synaptic membranes from rat substantia nigra. L-[3H]-GLU binding to the membrane preparations occurred in a reversible and saturable way. The specific binding was stimulated by the presence of CaCl2 and was reduced by freezing and thawing the membranes. Scatchard analysis of the saturation isotherms yielded a non-linear plot suggesting that the binding reaction does not occur through a simple biomolecular association. Assuming non-interacting binding sites, a high (KD1, 139 nM; Bmax1, 3.5 pmoles/mg protein) and a low (KD2, 667 nM; Bmax2, 15.1 pmoles/mg protein) affinity L-[3H]-GLU binding site were obtained. The kinetics of dissociation of bound L-[3H]-GLU was biphasic; the respective dissociation rate constant (k-1) being 0.20 min-1 and 0.013 min-1. A series of amino acid receptor agonists and antagonists were tested as inhibitors of L-[3H]-GLU specific binding. Quisqualic acid, L-GLU and D-.alpha.-aminoadipate (D-.alpha.-AA) were the most potent inhibitors. DL-2-amino-4-phosphonobutyrate (APB), N-Methyl-D-aspartate (NMDA) and D-GLU were moderate inhibitors, whereas diaminopimelic acid (DAPA) and glutamate diethyl ester (GDEE) exhibited the lowest relative potency. Kainic acid (KA), .gamma.-aminobutyric acid (GABA) and bicuculline were not able to modify at any concentration used the specific binding of L-[3H]-GLU. These data demonstrate the presence of specific GLU binding sites in synaptic structures at substantia nigra level and support the idea that excitatory amino acids may play a role in synaptic transmission in this brain region.
dc.fuente.origenWOS
dc.identifier.issn0197-5110
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99638
dc.identifier.wosidWOS:A1986H158100001
dc.issue.numero5-6
dc.language.isoen
dc.pagina.final360
dc.pagina.inicio339
dc.revistaJournal of receptor research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSPECIFIC BINDING OF L-[H-3]-GLUTAMIC ACID TO RAT SUBSTANTIA-NIGRA SYNAPTIC-MEMBRANES
dc.typeartículo
dc.volumen6
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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