<i>Helicobacter pylori</i> HopE and HopV porins present scarce expression among clinical isolates

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dc.contributor.authorLienlaf, Maritza
dc.contributor.authorPablo Morales, Juan
dc.contributor.authorInes Diaz, Maria
dc.contributor.authorDiaz, Rodrigo
dc.contributor.authorBruce, Elsa
dc.contributor.authorSiegel, Freddy
dc.contributor.authorLeon, Gloria
dc.contributor.authorHarris, Paul R.
dc.contributor.authorVenegas, Alejandro
dc.date.accessioned2025-01-21T00:06:52Z
dc.date.available2025-01-21T00:06:52Z
dc.date.issued2010
dc.description.abstractAIM: To evaluate how widely Helicobacter pylori (H. pylori) HopE and HopV porins are expressed among Chilean isolates and how seroprevalent they are among infected patients in Chile.
dc.description.abstractMETHODS: H. pylori hopE and hopV genes derived from strain CHCTX-1 were cloned by polymerase chain reaction (PCR), sequenced and expressed in Escherichia coli (E colt) AD494 (DE3). Gel-purified porins were used to prepare polyclonal antibodies. The presence of both genes was tested by PCR in a collection of H. pylori clinical isolates and their expression was detected in lysates by immunoblotting. Immune responses against HopE, HopV and other H, pylori antigens in sera from infected and non-infected patients were tested by Western blotting using these sera as first antibody on recombinant H. pylori antigens.
dc.description.abstractRESULTS: PCR and Western blotting assays revealed that 60 and 82 out of 130 Chilean isolates carried hopE and hopV genes, respectively, but only 16 and 9, respectively, expressed these porins. IgG serum immunoreactivity evaluation of 69 H, pylori-infected patients revealed that HopE and HopV were infrequently recognized (8.7% and 10.1% respectively) compared to H, pylori VacA (68.1%) and CagA (59.5%) antigens. Similar values were detected for IgA serum immunoreactivity against HopE (11.6%) and HopV (10.5%) although lower values for VacA (42%) and CagA (17.4%) were obtained when compared to the IgG response.
dc.description.abstractCONCLUSION: A scarce expression of HopE and HopV among Chilean isolates was found, in agreement with the infrequent seroconversion against these antigens when tested in infected Chilean patients. (C) 2010 Baishideng. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.3748/wjg.v16.i3.320
dc.identifier.eissn2219-2840
dc.identifier.issn1007-9327
dc.identifier.urihttps://doi.org/10.3748/wjg.v16.i3.320
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95606
dc.identifier.wosidWOS:000273884700007
dc.issue.numero3
dc.language.isoen
dc.pagina.final329
dc.pagina.inicio320
dc.revistaWorld journal of gastroenterology
dc.rightsacceso restringido
dc.subjectHelicobacter pylori
dc.subjectGene expression
dc.subjectHopE and HopV porins
dc.subjectAntigens
dc.subjectImmune response
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.title<i>Helicobacter pylori</i> HopE and HopV porins present scarce expression among clinical isolates
dc.typeartículo
dc.volumen16
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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