Cell Wall-Degrading Enzymes Enlarge the Pore Size of Intervessel Pit Membranes in Healthy and Xylella fastidiosa-Infected Grapevines

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dc.contributor.authorPerez Donoso, Alonso G.
dc.contributor.authorSun, Qiang
dc.contributor.authorRoper, M. Caroline
dc.contributor.authorGreve, L. Carl
dc.contributor.authorKirkpatrick, Bruce
dc.contributor.authorLabavitch, John M.
dc.date.accessioned2024-01-10T12:04:34Z
dc.date.available2024-01-10T12:04:34Z
dc.date.issued2010
dc.description.abstractThe pit membrane (PM) is a primary cell wall barrier that separates adjacent xylem water conduits, limiting the spread of xylem-localized pathogens and air embolisms from one conduit to the next. This paper provides a characterization of the size of the pores in the PMs of grapevine (Vitis vinifera). The PM porosity (PMP) of stems infected with the bacterium Xylella fastidiosa was compared with the PMP of healthy stems. Stems were infused with pressurized water and flow rates were determined; gold particles of known size were introduced with the water to assist in determining the size of PM pores. The effect of introducing trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA), oligogalacturonides, and polygalacturonic acid into stems on water flux via the xylem was also measured. The possibility that cell wall-degrading enzymes could alter the pore sizes, thus facilitating the ability of X. fastidiosa to cross the PMs, was tested. Two cell wall-degrading enzymes likely to be produced by X. fastidiosa (polygalactuoronase and endo-1,4-beta-glucanase) were infused into stems, and particle passage tests were performed to check for changes in PMP. Scanning electron microscopy of control and enzyme-infused stem segments revealed that the combination of enzymes opened holes in PMs, probably explaining enzyme impacts on PMP and how a small X. fastidiosa population, introduced into grapevines by insect vectors, can multiply and spread throughout the vine and cause Pierce's disease.
dc.description.funderPierce's Disease Control Program
dc.description.funderCalifornia Department of Food and Agriculture
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1104/pp.109.148791
dc.identifier.eissn1532-2548
dc.identifier.issn0032-0889
dc.identifier.pubmedidMEDLINE:20107028
dc.identifier.urihttps://doi.org/10.1104/pp.109.148791
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75835
dc.identifier.wosidWOS:000276329500049
dc.information.autorucAgronomía e Ing. Forestal;Pérez-Donoso A;S/I;91787
dc.information.autorucS/I; , ; S/I; 4
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final1759
dc.pagina.inicio1748
dc.publisherAMER SOC PLANT BIOLOGISTS
dc.revistaPLANT PHYSIOLOGY
dc.rightsacceso abierto
dc.subjectPIERCES DISEASE BACTERIUM
dc.subjectHYDRAULIC CONDUCTANCE
dc.subjectPASSIVE MECHANISM
dc.subjectRIPENING FRUIT
dc.subjectPATHOGEN
dc.subjectETHYLENE
dc.subjectPOLYGALACTURONASE
dc.subjectPOLYSACCHARIDE
dc.subjectPURIFICATION
dc.subjectRESISTANT
dc.titleCell Wall-Degrading Enzymes Enlarge the Pore Size of Intervessel Pit Membranes in Healthy and Xylella fastidiosa-Infected Grapevines
dc.typeartículo
dc.volumen152
sipa.codpersvinculados91787
sipa.codpersvinculados4
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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