PLATELETS ARE THE PRIMARY SOURCE OF AMYLOID BETA-PEPTIDE IN HUMAN BLOOD

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dc.contributor.authorCHEN, M
dc.contributor.authorINESTROSA, NC
dc.contributor.authorROSS, GS
dc.contributor.authorFERNANDEZ, HL
dc.date.accessioned2025-01-21T01:34:32Z
dc.date.available2025-01-21T01:34:32Z
dc.date.issued1995
dc.description.abstractThe main component of Alzheimer's disease (AD) amyloid deposits is amyloid beta-peptide (A beta), a fragment of the larger amyloid precursor protein (APP). The cellular source of A beta is not known, but a circulatory origin has been postulated. We studied human blood from healthy individuals and found that platelets account for almost 90% of the total anti-A beta immunoreactivity detected in whole blood. Using reverse-phase HPLC, we identified a platelet peptide which corresponds to A beta by three criteria. (a) it shares a retention time with the synthetic A beta(1-40) peptide in two consecutive HPLC tests; (b) it interacts with two anti-A beta antibodies in separate ELISAs; and, (c) its partial N-terminal amino acid sequence closely matches that of A beta. The detection of this peptide in platelets indicates that, aside from the well-known non-amyloidogenic (secretory) pathway, the processing of APP in platelets from healthy individuals also involves an amyloidogenic pathway. These findings are consistent with the view that platelets are one of the major sources of A beta in the circulation. (C) 1995 Academic Press, Inc.
dc.fuente.origenWOS
dc.identifier.issn0006-291X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97571
dc.identifier.wosidWOS:A1995RN59400014
dc.issue.numero1
dc.language.isoen
dc.pagina.final103
dc.pagina.inicio96
dc.revistaBiochemical and biophysical research communications
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePLATELETS ARE THE PRIMARY SOURCE OF AMYLOID BETA-PEPTIDE IN HUMAN BLOOD
dc.typeartículo
dc.volumen213
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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