Heterologous expression of laccase cDNA from <i>Ceriporiopsis subvermispora</i> yields copper-activated apoprotein and complex isoform patterns

dc.contributor.authorLarrondo, LF
dc.contributor.authorAvila, M
dc.contributor.authorSalas, L
dc.contributor.authorCullen, D
dc.contributor.authorVicuña, R
dc.date.accessioned2025-01-21T01:09:27Z
dc.date.available2025-01-21T01:09:27Z
dc.date.issued2003
dc.description.abstractAnalysis of genomic clones encoding a putative laccase in homokaryon strains of Ceriporiopsis subvermispora led to the identification of an allelic variant of the previously described Ics-1 gene. A cDNA clone corresponding to this gene was expressed in Aspergillus nidulans and in Aspergillus niger. Enzyme assays and Western blots showed that both hosts secreted active laccase. Relative to the isozymic forms of the native C. subvermispora enzyme, the A. niger-produced laccase had a higher molecular mass and gave a single band on IEF gels. In contrast, A. nidulans transformants secreted several isoforms remarkably similar to those of the native system. Considered together with previously reported Southern blots and protein sequencing, expression in A. nidulans supports the view that C. subvermispora has a single laccase gene and that multiple isoforms result from post-translational processes. In addition, several lines of evidence strongly suggest that under copper limitation, A. nidulans secretes apoprotein which can be reconstituted by a short incubation with Cu(I) and to a lesser extent with Cu(II).
dc.fuente.origenWOS
dc.identifier.doi10.1099/mic.0.26147-0
dc.identifier.issn1350-0872
dc.identifier.urihttps://doi.org/10.1099/mic.0.26147-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96584
dc.identifier.wosidWOS:000183033000010
dc.language.isoen
dc.pagina.final1182
dc.pagina.inicio1177
dc.revistaMicrobiology-sgm
dc.rightsacceso restringido
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleHeterologous expression of laccase cDNA from <i>Ceriporiopsis subvermispora</i> yields copper-activated apoprotein and complex isoform patterns
dc.typeartículo
dc.volumen149
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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