MOLECULAR TOOLS FOR RAPID AND ACCURATE DETECTION OF BLACK TRUFFLE (<i>Tuber melanosporum</i> Vitt.) IN INOCULATED NURSERY PLANTS AND COMMERCIAL PLANTATIONS IN CHILE

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dc.contributor.authorCordero, Cecilia
dc.contributor.authorCaceres, Pablo
dc.contributor.authorGonzalez, Gloria
dc.contributor.authorQuiroz, Karla
dc.contributor.authorBravo, Carmen
dc.contributor.authorRamirez, Ricardo
dc.contributor.authorCaligari, Peter D. S.
dc.contributor.authorCarrasco, Basilio
dc.contributor.authorGarcia-Gonzales, Rolando
dc.date.accessioned2025-01-21T00:01:19Z
dc.date.available2025-01-21T00:01:19Z
dc.date.issued2011
dc.description.abstractTruffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5'-GTAACGATAAAGGCCATCTATAGG-3') and ADL3 (5'-CGTTTTTCCTGAACTCTTCATCAC-3'), where a restriction fragment of 160 bp specific for T. melanosporum was generated, which allows the discrimination of this species from the rest of the species belonging to the Tuber sp. genus. Direct detection of T. melanosporum in one step was also obtained by polymerase chain reaction (PCR) from total DNA isolated from mycorrhized roots and with the primers ITSML (5'-TGGCCATGTGTCAGATTTAGTA-3') and ITSLNG (5'-TGATATGCTTAAGTTCAGCGGG-3'), generating a single amplicon of 440 bp. The molecular detection of T. melanosporum by the methods presented here will allow the rapid and accurate detection of mycorrhization of trees, both under nursery and field conditions. This technology will also provide more security to farmers by controlling the quality of the mycorrhized trees they will plant and also by following the mycorrhization status of established orchards.
dc.fuente.origenWOS
dc.identifier.issn0718-5839
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95383
dc.identifier.wosidWOS:000295893200022
dc.issue.numero3
dc.language.isoen
dc.pagina.final494
dc.pagina.inicio488
dc.revistaChilean journal of agricultural research
dc.rightsacceso restringido
dc.subjectTuber melanosporum
dc.subjectectomycorrhizas
dc.subjectmolecular markers
dc.subjectPCR
dc.subjectCAPS
dc.subject.ods02 Zero Hunger
dc.subject.ods15 Life on Land
dc.subject.odspa02 Hambre cero
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleMOLECULAR TOOLS FOR RAPID AND ACCURATE DETECTION OF BLACK TRUFFLE (<i>Tuber melanosporum</i> Vitt.) IN INOCULATED NURSERY PLANTS AND COMMERCIAL PLANTATIONS IN CHILE
dc.typeartículo
dc.volumen71
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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