Low abundance of Mfn2 protein correlates with reduced mitochondria-SR juxtaposition and mitochondrial cristae density in human men skeletal muscle: Examining organelle measurements from TEM images

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dc.contributor.authorCastro-Sepulveda, Mauricio
dc.contributor.authorFernandez-Verdejo, Rodrigo
dc.contributor.authorTunon-Suarez, Mauro
dc.contributor.authorMorales-Zuniga, Jorge
dc.contributor.authorTroncoso, Mayarling
dc.contributor.authorJannas-Vela, Sebastian
dc.contributor.authorZbinden-Foncea, Hermann
dc.date.accessioned2025-01-20T23:51:23Z
dc.date.available2025-01-20T23:51:23Z
dc.date.issued2021
dc.description.abstractThe role of mitofusin 2 (Mfn2) in the regulation of skeletal muscle (SM) mitochondria-sarcoplasmic (SR) juxtaposition, mitochondrial morphology, mitochondrial cristae density (MCD), and SM quality has not been studied in humans. In in vitro studies, whether Mfn2 increases or decreases mitochondria-SR juxtaposition remains controversial. Transmission electron microscopy (TEM) images are commonly used to measure the organelle juxtaposition, but the measurements are performed "by-hand," thus potentially leading to between-rater differences. The purposes of this study were to: (1) examine the repeatability and reproducibility of mitochondrial-SR juxtaposition measurement from TEM images of human SM between three raters with different experience and (2) compare the mitochondrial-SR juxtaposition, mitochondrial morphology, MCD (stereological-method), and SM quality (cross-sectional area [CSA] and the maximum voluntary contraction [MVC]) between subjects with high abundance (Mfn2-HA; n = 6) and low abundance (Mfn2-LA; n = 6) of Mfn2 protein. The mitochondria-SR juxtaposition had moderate repeatability and reproducibility, with the most experienced raters showing the best values. There were no differences between Mfn2-HA and Mfn2-LA groups in mitochondrial size, distance from mitochondria to SR, CSA, or MVC. Nevertheless, the Mfn2-LA group showed lower mitochondria-SR interaction, MCD, and VO2max. In conclusion, mitochondrial-SR juxtaposition measurement depends on the experience of the rater, and Mfn2 protein seems to play a role in the metabolic control of human men SM, by regulating the mitochondria-SR interaction.
dc.description.funderUniversidad Finis Terrae
dc.fuente.origenWOS
dc.identifier.doi10.1096/fj.202002615RR
dc.identifier.eissn1530-6860
dc.identifier.issn0892-6638
dc.identifier.urihttps://doi.org/10.1096/fj.202002615RR
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94804
dc.identifier.wosidWOS:000635216100050
dc.issue.numero4
dc.language.isoen
dc.revistaFaseb journal
dc.rightsacceso restringido
dc.subjectMICOS complex
dc.subjectmitochondria dynamics
dc.subjectorganelle communication
dc.subjectrepeatability
dc.subjectreproducibility
dc.subjecttransmission electron microscopy
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLow abundance of Mfn2 protein correlates with reduced mitochondria-SR juxtaposition and mitochondrial cristae density in human men skeletal muscle: Examining organelle measurements from TEM images
dc.typeartículo
dc.volumen35
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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