Open educational resources for distributed hands-on teaching in molecular biology

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dc.article.number0327975
dc.catalogadorgrr
dc.contributor.authorCerda Rojas, Ariel Patricio
dc.contributor.author Castillo-Navarrete, Juan Luis
dc.contributor.authorAravena Lazo, Alejandro Daniel
dc.contributor.authorZapata Romero, Valentina Paz
dc.contributor.authorArce Medina, Anibal Andres
dc.contributor.authorAraya, Wladimir
dc.contributor.authorGallardo, Domingo
dc.contributor.authorAviles, Javiera
dc.contributor.author Quero, Francisco
dc.contributor.author Nuñez, Isaac
dc.contributor.authorMatute, Tamara
dc.contributor.authorNavarro, Felipe
dc.contributor.authorBlanco Zepeda, Marta Victoria
dc.contributor.authorVelozo Caballero, Sebastian Ignacio
dc.contributor.authorRodriguez, Sebastian
dc.contributor.authorAguilera, Sebastian
dc.contributor.authorChateau Gannon, Francisco
dc.contributor.authorOlivares Donoso, Ruby Carolina
dc.contributor.author Ramirez-Sarmiento, Cesar
dc.contributor.author Federici, Fernan
dc.date.accessioned2025-08-27T20:33:13Z
dc.date.available2025-08-27T20:33:13Z
dc.date.issued2025
dc.descriptionhttps://doi.org/10.1371/journal.pone.0327975This work was supported by the National Agency for Research and Development (ANID) through the ANID Millennium Science Initiative Program (ICN17_022), Fondo de Desarrollo Científico y Tecnológico (FONDECYT 1201684 awarded to C.A. Ramírez-Sarmiento, FONDECYT Regular 1211218 & FONDECYT Regular 1241452 to Fernan Federici, and an International Cooperation Program with Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (ANID-CONCYTEC covbio0012 awarded to F. Federici and C.A. Ramírez-Sarmiento) and GOSH funding to G. Aidelberg, F. Quero and A. B. Lindner. There was no additional external funding received for this study.Competing interests: The
dc.description.abstractThe urgent need to develop a more equitable bioeconomy has positioned biotechnology capacity building at the forefront of international priorities. However, in many educational institutions, particularly in low- and middle-income countries, this remains a major challenge due to limited access to reagents, equipment, and technical documentation. In this work, we describe Open Educational Resources (OER) composed of locally produced biological reagents, open source hardware and free software to teach fundamental techniques in biotechnology such as LAMP DNA amplification, RT-PCR RNA detection, enzyme kinetics and fluorescence imaging. The use of locally produced reagents and devices reduces costs by up to one order of magnitude. During the pandemic lockdowns, these tools were distributed nationwide to students’ homes as a lab-in-a-box for remote teaching of molecular biology. To test their performance, a total of 93 undergraduate students tested these resources during a biochemistry practical course. 27 out of 31 groups (~87%) successfully achieved the objectives of the PCR activity, while 28 out of 31 groups (~90%) correctly identified the target using LAMP reactions. To assess the potential application in secondary school, we organized three workshops for high school teachers from different institutions across Chile and performed an anonymous questionnaire, obtaining a strong agreement on how these OER broaden teachers’ perspectives on the techniques and facilitate the teaching of molecular biology topics. This effort was made possible through a close collaboration with open source technology advocates and members of DIYbio communities, whose work is paving the way for low-cost training in biology. All the protocols and design files are available under open source licenses
dc.fechaingreso.objetodigital2025-08-27
dc.format.extent23 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1371/journal. pone.0327975
dc.identifier.eissn1932-6203
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105324
dc.identifier.urihttps://doi.org/10.1371/journal. pone.0327975
dc.information.autorucFacultad de Ciencias Biológicas; Cerda Rojas, Ariel Patricio; 0009-0007-3040-4849; 250244
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Aravena Lazo, Alejandro Daniel; S/I; 221004
dc.information.autorucFacultad de Ciencias Biológicas; Zapata Romero, Valentina Paz; S/I; 215463
dc.information.autorucFacultad de Ciencias Biológicas; Arce Medina, Anibal Andres; 0000-0001-8134-6385; 178308
dc.information.autorucFacultad de Ciencias Biológicas; Blanco Zepeda, Marta Victoria; S/I; 205486
dc.information.autorucFacultad de Ciencias Biológicas; Velozo Caballero, Sebastian Ignacio; S/I; 247425
dc.information.autorucEscuela de Arquitectura; Chateau Gannon, Francisco; 0000-0002-1082-8851; 4049
dc.information.autorucFacultad de Educación; Olivares Donoso, Ruby Carolina; 0000-0001-7781-7076; 225058
dc.issue.numero8
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaPLoS ONE
dc.rightsacceso abierto
dc.rights.licenseCC BY 4.0 Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc370
dc.subject.deweyEducaciónes_ES
dc.subject.ods04 Quality education
dc.subject.odspa04 Educación de calidad
dc.titleOpen educational resources for distributed hands-on teaching in molecular biology
dc.typeartículo
dc.volumen 20
sipa.codpersvinculados250244
sipa.codpersvinculados221004
sipa.codpersvinculados215463
sipa.codpersvinculados178308
sipa.codpersvinculados205486
sipa.codpersvinculados247425
sipa.codpersvinculados4049
sipa.codpersvinculados225058
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