METABOLISM OF CHLORINATED GUAIACOLS BY A GUAIACOL-DEGRADING ACINETOBACTER-JUNII STRAIN

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dc.contributor.authorGONZALEZ, B
dc.contributor.authorACEVEDO, C
dc.contributor.authorBREZNY, R
dc.contributor.authorJOYCE, T
dc.date.accessioned2025-01-23T19:21:37Z
dc.date.available2025-01-23T19:21:37Z
dc.date.issued1993
dc.description.abstractThe metabolism of chlorinated guaiacols by a pure bacterial strain identified by its ability to use guaiacol as the sole carbon and energy source was studied. This strain, identified as Acinetobacter junii 5ga, was unable to grow on several chlorinated guaiacols and catechols. However, strain 5ga grown on guaiacol degraded 4- and 5-chloroguaiacol and 4,5-dichloroguaiacol. Under the same conditions, these cells did not degrade 6-chloroguaiacol, 4,6-dichloroguaiacol, 4,5,6-trichloroguaiacol, or tetrachlorognuiacol, suggesting that the substitution at the 6 position in the ring prevents metabolism of the compound. Degradation of 4-chloroguaiacol was dependent on the initial ratio between the chlorinated compound and viable cells. Transient formation of chlorocatechols resulting from incubation of cells with 4-chloroguaiacol or 4,5-dichloroguaiacol was suggested by UV spectroscopy. Gas chromatography analyses of samples from cultures of strain 5ga grown on guaiacol and incubated with 4- and 4,5-dichloroguaiacol confirmed the presence of 4-chlorocatechol and 4,5-dichlorocatechol, respectively. The formation of the latter was corroborated by gas chromatography-mass spectrometry. Thus, this strain is able to initiate metabolism of specific chlorinated guaiacols by O-demethylation. The starting chlorinated guaiacols and their 0-demethylated metabolites inhibited the growth of A.junii 5ga on guaiacol.
dc.fuente.origenWOS
dc.identifier.issn0099-2240
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98821
dc.identifier.wosidWOS:A1993MA35300038
dc.issue.numero10
dc.language.isoen
dc.pagina.final3429
dc.pagina.inicio3424
dc.revistaApplied and environmental microbiology
dc.rightsacceso restringido
dc.titleMETABOLISM OF CHLORINATED GUAIACOLS BY A GUAIACOL-DEGRADING ACINETOBACTER-JUNII STRAIN
dc.typeartículo
dc.volumen59
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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