The PcACE1 transcription factor from <i>Phanerochaete chrysosporium</i> contains a Cys- and Ser-rich transactivation domain

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dc.contributor.authorBarham, Feiruz
dc.contributor.authorRoman, Patricia
dc.contributor.authorBull, Paulina
dc.date.accessioned2025-01-21T00:03:10Z
dc.date.available2025-01-21T00:03:10Z
dc.date.issued2011
dc.description.abstractTranscription factor Ace1 from Saccharomyces cerevisiae regulates the expression of target genes when the copper concentration reaches 200 mu M levels. We are studying the ortholog of Ace1 from fungus Phanerochaete chrysosporium PcACE1, isolated by complementation in yeast. In this report we show the localization of the transactivation region of PcACE1. Different PcACE1 fragments were ligated in frame to the GAL4 DNA-binding domain by site-directed mutagenesis in a suitable yeast expression vector. Transformation of an appropriate Saccharomyces cerevisiae strain was used as host. This strain contains the fusion GAL1:lacZ in its genome under the control of promoter sequences recognized by GAL4. Finally, we measured beta-galactosidase activity in each yeast clone. The activation of the reporter gene is proportional to the transactivation capacity of the transcription factor PcACE1.
dc.description.abstractThe results obtained indicate that PcACE1 transactivation domain is located in the carboxy terminal half and contains an array of cysteines in the form of Cys-X-Cys and Cys-chi(2)-Cys and a 60% of Ser. Therefore, these results show that this type of Cys motif can function as transcription activating domain not only in transcription factors that respond to minimal copper concentrations but also in those that respond to high copper concentrations. This is the first transactivation domain reported in a basidiomycete fungus.
dc.description.funderFONDECYT
dc.fuente.origenWOS
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95452
dc.identifier.wosidWOS:000299190500006
dc.issue.numero4
dc.language.isoen
dc.pagina.final355
dc.pagina.inicio351
dc.revistaBiological research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe PcACE1 transcription factor from <i>Phanerochaete chrysosporium</i> contains a Cys- and Ser-rich transactivation domain
dc.typeartículo
dc.volumen44
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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