Functional characterization of <i>Citrus macrophylla BOR1</i> as a boron transporter

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dc.contributor.authorCanon, Paola
dc.contributor.authorAquea, Felipe
dc.contributor.authorRodriguez-Hoces de la Guardia, Amparo
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-24T00:08:06Z
dc.date.available2025-01-24T00:08:06Z
dc.date.issued2013
dc.description.abstractPlants have evolved to develop an efficient system of boron uptake and transport using a range of efflux carriers named BOR proteins. In this work we isolated and characterized a boron transporter of citrus (Citrus macrophylla), which was named CmBOR1 for its high homology to AtBOR1. CmBOR1 has 4403bp and 12 exons. Its coding region has 2145bp and encodes for a protein of 714 amino acids. CmBOR1 possesses the molecular features of BORs such as an anion exchanger domain and the presence of 10 transmembrane domains. Functional analysis in yeast indicated that CmBOR1 has an efflux boron transporter activity, and transformants have increased tolerance to excess boron. CmBOR1 is expressed in leaves, stem and flowers and shows the greatest accumulation in roots. The transcript accumulation was significantly increased under boron deficiency conditions in shoots. In contrast, the accumulation of the transcript did not change in boron toxicity conditions. Finally, we observed that constitutive expression of CmBOR1 was able to increase tolerance to boron deficiency conditions in Arabidopsis thaliana, suggesting that CmBOR1 is a xylem loading boron transporter. Based on these results, it was determined that CmBOR1 encodes a boric acid/borate transporter involved in tolerance to boron deficiency in plants.
dc.fuente.origenWOS
dc.identifier.doi10.1111/ppl.12037
dc.identifier.eissn1399-3054
dc.identifier.issn0031-9317
dc.identifier.urihttps://doi.org/10.1111/ppl.12037
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101946
dc.identifier.wosidWOS:000325623800004
dc.issue.numero3
dc.language.isoen
dc.pagina.final339
dc.pagina.inicio329
dc.revistaPhysiologia plantarum
dc.rightsacceso restringido
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleFunctional characterization of <i>Citrus macrophylla BOR1</i> as a boron transporter
dc.typeartículo
dc.volumen149
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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