Interactions between Core Elements of the <i>Botrytis cinerea</i> Circadian Clock Are Modulated by Light and Different Protein Domains

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dc.contributor.authorRojas, Vicente
dc.contributor.authorSalinas, Francisco
dc.contributor.authorRomero, Andres
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.date.accessioned2025-01-20T21:06:54Z
dc.date.available2025-01-20T21:06:54Z
dc.date.issued2022
dc.description.abstractBotrytis cinerea possesses a complex light-sensing system composed of eleven photoreceptors. In B. cinerea, bcwcl1 encodes for the BcWCL1 protein, the orthologue of the blue-light photoreceptor WC-1 from Neurospora crassa. The functional partner of BcWCL1 is the BcWCL2 protein, both interacting in the nucleus and forming the B. cinerea white collar complex (BcWCC). This complex is required for photomorphogenesis and circadian regulation. However, no molecular evidence shows a light-dependent interaction between the BcWCC components or light-sensing capabilities in BcWCL1. In this work, by employing a yeast two-hybrid system that allows for the in vivo analysis of protein-protein interactions, we confirm that BcWCL1 and BcWCL2 interact in the absence of light as well as upon blue-light stimulation, primarily through their PAS (Per-Arnt-Sim) domains. Deletion of the PAS domains present in BcWCL1 (BcWCL1(PAS increment )) or BcWCL2 (BcWCL2(PAS increment )) severely impairs the interaction between these proteins. Interestingly, the BcWCL1(PAS increment ) protein shows a blue-light response and interacts with BcWCL2 or BcWCL2(PAS increment ) upon light stimulation. Finally, we demonstrate that BcWCL1 and BcWCL1(PAS increment ) respond to blue light by introducing a point mutation in the photoactive cysteine, confirming that both proteins are capable of light sensing. Altogether, the results revealed the complexity of protein-protein interactions occurring between the core elements of the B. cinerea circadian clock.
dc.fuente.origenWOS
dc.identifier.doi10.3390/jof8050486
dc.identifier.eissn2309-608X
dc.identifier.urihttps://doi.org/10.3390/jof8050486
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93379
dc.identifier.wosidWOS:000802490900001
dc.issue.numero5
dc.language.isoen
dc.revistaJournal of fungi
dc.rightsacceso restringido
dc.subjectBotrytis cinerea
dc.subjectphotoreceptors
dc.subjectyeast
dc.subjectPAS and LOV domains
dc.subjectoptogenetics
dc.titleInteractions between Core Elements of the <i>Botrytis cinerea</i> Circadian Clock Are Modulated by Light and Different Protein Domains
dc.typeartículo
dc.volumen8
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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