Expression of biomineralization-related ion transport genes in <i>Emiliania huxleyi</i>

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dc.contributor.authorMackinder, Luke
dc.contributor.authorWheeler, Glen
dc.contributor.authorSchroeder, Declan
dc.contributor.authorvon Dassow, Peter
dc.contributor.authorRiebesell, Ulf
dc.contributor.authorBrownlee, Colin
dc.date.accessioned2025-01-21T00:00:03Z
dc.date.available2025-01-21T00:00:03Z
dc.date.issued2011
dc.description.abstractBiomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO(2) levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is down-regulated in calcifying cells. The data provide strong evidence that these genes play key roles in E. huxleyi biomineralization. Based on the gene expression data and the current literature a working model for biomineralization-related ion transport in coccolithophores is presented.
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1462-2920.2011.02561.x
dc.identifier.eissn1462-2920
dc.identifier.issn1462-2912
dc.identifier.urihttps://doi.org/10.1111/j.1462-2920.2011.02561.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95322
dc.identifier.wosidWOS:000298843800015
dc.issue.numero12
dc.language.isoen
dc.pagina.final3265
dc.pagina.inicio3250
dc.revistaEnvironmental microbiology
dc.rightsacceso restringido
dc.subject.ods13 Climate Action
dc.subject.ods14 Life Below Water
dc.subject.odspa13 Acción por el clima
dc.subject.odspa14 Vida submarina
dc.titleExpression of biomineralization-related ion transport genes in <i>Emiliania huxleyi</i>
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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