Increased expression of c-<i>rel</i>, from the NF-κB/Rel family, in T cells from patients with systemic lupus erythematosus

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dc.contributor.authorBurgos, P
dc.contributor.authorMetz, C
dc.contributor.authorBull, P
dc.contributor.authorPincheira, R
dc.contributor.authorMassardo, L
dc.contributor.authorErrázuriz, C
dc.contributor.authorBono, MR
dc.contributor.authorJacobelli, S
dc.contributor.authorGonzález, A
dc.date.accessioned2025-01-21T01:31:40Z
dc.date.available2025-01-21T01:31:40Z
dc.date.issued2000
dc.description.abstractObjective. To explore the role of the NF-kappa B/Rel transcription factor family in autoimmunity, we investigated whether peripheral blood mononuclear cells (PBMC) and T cells from the blood of patients with systemic lupus erythematosus (SLE) exhibit abnormal expression of c-rel, both when recently isolated and/or during in vitro activation.
dc.description.abstractMethods. Total RNA and protein extracts were prepared from PBMC and T cells isolated by immunoadsorption with magnetic beads. The relative concentrations of c-rel mRNA and of c-Rel protein were determined by semiquantitative assays of competitive reverse transcriptase-polymerase chain reaction and chemiluminescent immunoblots, respectively. Activity of NF-kappa B/Rel was studied by electrophoretic mobility shift assay of nuclear extracts.
dc.description.abstractResults, Significantly increased levels of c-rel mRNA were found (1) in PBMC from SLE patients (n = 48; p < 0.0000001), even during inactive disease (n = 11; p < 0.001), compared to controls (n = 54), and (2) in T cells isolated from a subgroup of these: patients (n = 11; p < 0.00003) and controls (n = 12). c-Rel protein was found increased in the cytosol but not in the nucleus of PBMC of patients with SLE (n = 12; p < 0.02) compared to controls (n = 12). No evidence of NF-kappa B/Rel nuclear activity was detected. In vitro stimulation of T cells by incubating PBMC with concanavalin A showed that less c-Rel entered the nucleus in lupus cells than healthy cells, correlating with lower interleukin 2 production. However, the same stimulating conditions provoked an increase in c-rel mRNA to higher levels in lupus cells from 3 patients compared with 2 controls. Increased levels of both I kappa B alpha and I kappa B beta could account for c-Rel cytosolic retention.
dc.description.abstractConclusion. Our data suggest that T cells from patients with SLE possess altered regulatory mechanisms of c-rel expression and nuclear import that might potentially determine conditions for developing autoimmunity. Other cells present in the PBMC could also be affected.
dc.fuente.origenWOS
dc.identifier.issn0315-162X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97116
dc.identifier.wosidWOS:000084778000021
dc.issue.numero1
dc.language.isoen
dc.pagina.final127
dc.pagina.inicio116
dc.revistaJournal of rheumatology
dc.rightsacceso restringido
dc.subjectNF-kappa B/Rel
dc.subjectc-rel
dc.subjectautoimmunity
dc.subjectsystemic lupus erythematosus
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIncreased expression of c-<i>rel</i>, from the NF-κB/Rel family, in T cells from patients with systemic lupus erythematosus
dc.typeartículo
dc.volumen27
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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