Vitamin E but not 17β-estradiol protects against vascular toxicity induced by β-amyloid wild type and the Dutch amyloid variant

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dc.contributor.authorMuñoz, FJ
dc.contributor.authorOpazo, C
dc.contributor.authorGil-Gómez, G
dc.contributor.authorTapia, G
dc.contributor.authorFernández, V
dc.contributor.authorValverde, MA
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:30:27Z
dc.date.available2025-01-21T01:30:27Z
dc.date.issued2002
dc.description.abstractAmyloid beta-peptide (Abeta) fibril deposition on cerebral vessels produces cerebral amyloid angiopathy that appears in the majority of Alzheimer's disease patients. An early onset of a cerebral amyloid angiopathy variant called hereditary cerebral hemorrhage with amyloidosis of the Dutch type is caused by a point mutation in Abeta yielding Abeta(Glu22-->Gln). The present study addresses the effect of amyloid fibrils from both wild-type and mutated Abeta on vascular cells, as well as the putative protective role of antioxidants on amyloid angiopathy. For this purpose, we studied the cytotoxicity induced by Abeta(1-40 Glu22-->Gln) and Abeta(1-40 wild-type) fibrils on human venule endothelial cells and rat aorta smooth muscle cells. We observed that Abeta(Glu22-->Gln) fibrils are more toxic for vascular cells than the wild-type fibrils. We also evaluated the cytotoxicity of Abeta fibrils bound with acetylcholinesterase (AChE), a common component of amyloid deposits. Abeta(1-40 wild-type)-AChE fibrillar complexes, similar to neuronal cells, resulted in an increased toxicity on vascular cells. Previous reports showing that antioxidants are able to reduce the toxicity of Abeta fibrils on neuronal cells prompted us to test the effect of vitamin E, vitamin C, and 17beta-estradiol on vascular damage induced by Abeta(wild-type) and Abeta(Glu22-->Gln). Our data indicate that vitamin E attenuated significantly the Abeta-mediated cytotoxicity on vascular cells, although 17beta-estradiol and vitamin C failed to inhibit the cytotoxicity induced by Abeta fibrils.
dc.fuente.origenWOS
dc.identifier.issn0270-6474
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96760
dc.identifier.wosidWOS:000174841300012
dc.issue.numero8
dc.language.isoen
dc.pagina.final3089
dc.pagina.inicio3081
dc.revistaJournal of neuroscience
dc.rightsacceso restringido
dc.subjectAlzheimer's disease
dc.subjectCAA
dc.subjectHCHWA-D
dc.subjectamyloid
dc.subjectvitamin E
dc.subject17 beta-estradiol
dc.subjectvitamin C
dc.subjectoxidative stress
dc.subjectacetylcholinesterase
dc.subjectendothelial cells
dc.subjectvascular smooth muscle cells
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleVitamin E but not 17β-estradiol protects against vascular toxicity induced by β-amyloid wild type and the Dutch amyloid variant
dc.typeartículo
dc.volumen22
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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