A basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system

Simple item page
dc.contributor.authorMoreno, RD
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:31:21Z
dc.date.available2025-01-21T01:31:21Z
dc.date.issued2000
dc.description.abstractProacrosin is the zymogen of acrosin, a serine protease localized in the acrosomal matrix of mammalian sperm. Proacrosin/acrosin binds to solubilized zona pellucida glycoproteins (ZPGs) and various polysulfates in a non-enzymatic mechanism. In addition, both polysulfates and ZPGs induce proacrosin activation once they bind to the polysulfate-binding domain (PSBD) of the enzyme. We show here that the peptide (43)IFMYHNNRRYHTCCGILL(60) inhibited the proacrosin activation induced by either fucoidan or ZPGs. In addition, the peptide was recognized by the monoclonal antibody C5F10, which is directed against the PSBD region. Our data suggest that the PSBD is composed of many "subsites" that may or may not interact with each other.
dc.fuente.origenWOS
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97044
dc.identifier.wosidWOS:000087239600008
dc.issue.numero6
dc.language.isoen
dc.pagina.final1542
dc.pagina.inicio1536
dc.revistaBiology of reproduction
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system
dc.typeartículo
dc.volumen62
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas