Disparate effects of estradiol on egg transport and oviductal protein synthesis in mated and cyclic rats

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dc.contributor.authorOrihuela, PA
dc.contributor.authorRíos, M
dc.contributor.authorCroxatto, HB
dc.date.accessioned2025-01-21T01:30:41Z
dc.date.available2025-01-21T01:30:41Z
dc.date.issued2001
dc.description.abstractPreviously, we found that the dose of estradiol (E-2) require to accelerate egg transport increases 5- to 10-fold, in mated compared to cyclic rats. Here we examined protein synthesis in the oviduct of mated and cyclic rats following a single injection of E-2 known to accelerate oviductal egg transport or after concomitant treatment with progesterone (P-4) known to block this acceleration. On Day 1 of the cycle or pregnancy, E-2, P-4, or E-2 + P-4 were injected s.c., and 4 h later oviducts were removed and incubated for 8 h in medium with S-35-methionine. Tissue proteins were separated by SDS-PAGE, and protein bands were quantitated by fluorography and densitometry. In mated rats, E-2 and P-4 increased different protein bands and P-4 did not affect the fluorographic pattern induced by E-2. In contrast with mated rats, none of these treatments changed the fluorographic pattern of the oviductal proteins in cyclic rats. Estradiol-induced egg transport acceleration was then compared under conditions in which oviductal protein synthesis was suppressed. Mated and cyclic rats treated with equipotent doses of E-2 for accelerating egg transport also received actinomycin D (Act D) locally. Estradiol-induced oviductal egg loss was partially blocked by Act D in mated but had no effect in cyclic rats. We conclude that the oviduct of mated and cyclic rats differs in that only the former responds with increased protein synthesis to a pulse of exogenous E-2 and P-4 and requires an intact protein synthesis machinery in order to accelerate egg transport in response to E-2.
dc.fuente.origenWOS
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96859
dc.identifier.wosidWOS:000171320400033
dc.issue.numero4
dc.language.isoen
dc.pagina.final1237
dc.pagina.inicio1232
dc.revistaBiology of reproduction
dc.rightsacceso restringido
dc.subjectestradiol
dc.subjectgene regulation
dc.subjectmechanisms of hormone action
dc.subjectoviduct
dc.subjectprogesterone
dc.subjectsteroid hormones
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDisparate effects of estradiol on egg transport and oviductal protein synthesis in mated and cyclic rats
dc.typeartículo
dc.volumen65
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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