Using an embryo specific promoter to modify iron distribution pattern in Arabidopsis

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dc.contributor.authorFuenzalida, Marlene
dc.contributor.authorGomez, Maria Isabel
dc.contributor.authorFerrada, Evandro
dc.contributor.authorDiaz, Cristobal
dc.contributor.authorEscudero, Viviana
dc.contributor.authorGonzalez-Guerrero, Manuel
dc.contributor.authorJordana, Xavier
dc.contributor.authorRoschzttardtz, Hannetz
dc.date.accessioned2025-01-20T17:10:50Z
dc.date.available2025-01-20T17:10:50Z
dc.date.issued2024
dc.description.abstractIron is an essential micronutrient for life. During the development of the seed, iron accumulates during embryo maturation. In Arabidopsis thaliana, iron mainly accumulates in the vacuoles of only one cell type, the cell layer that surrounds provasculature in hypocotyl and cotyledons. Iron accumulation pattern in Arabidopsis is an exception in plant phylogeny, most part of the dicot embryos accumulate iron in several cell layers including cortex and, in some cases, even in protodermis. It remains unknown how does iron reach the internal cell layers of the embryo, and in particular, the molecular mechanisms responsible of this process. Here, we use transgenic approaches to modify the iron accumulation pattern in an Arabidopsis model. Using the SDH2-3 embryo-specific promoter, we were able to express VIT1 ectopically in both a wild type background and a mutant vit1 background lacking expression of this vacuolar iron transporter. These manipulations modify the iron distribution pattern in Arabidopsis from one cell layer to several cell layers, including protodermis, cortex cells, and the endodermis. Interestingly, total seed iron content was not modified compared with the wild type, suggesting that iron distribution in embryos is not involved in the control of the total iron amount accumulated in seeds. This experimental model can be used to study the processes involved in iron distribution patterning during embryo maturation and its evolution in dicot plants.
dc.description.funderFONDECYT
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.plantsci.2023.111931
dc.identifier.eissn1873-2259
dc.identifier.issn0168-9452
dc.identifier.urihttps://doi.org/10.1016/j.plantsci.2023.111931
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91138
dc.identifier.wosidWOS:001134462200001
dc.language.isoen
dc.revistaPlant science
dc.rightsacceso restringido
dc.subjectIron
dc.subjectSeed
dc.subjectVIT1 ectopic expression
dc.subjectPerls/DAB staining
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleUsing an embryo specific promoter to modify iron distribution pattern in Arabidopsis
dc.typeartículo
dc.volumen339
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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