EFFECT OF PHENYTOIN ON CYTOSKELETAL PROTEIN-PHOSPHORYLATION AND NEURONAL STRUCTURE IN THE RAT SENSORY CORTEX

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dc.contributor.authorRUIZ, G
dc.contributor.authorFLORES, OG
dc.contributor.authorGONZALEZPLAZA, R
dc.contributor.authorINESTROSA, NC
dc.date.accessioned2025-01-23T19:26:02Z
dc.date.available2025-01-23T19:26:02Z
dc.date.issued1987
dc.description.abstractPhenytoin (PH) is commonly used as an anticonvulsant drug, and it causes several collateral effects including morphological changes in brain cortex neurons and teratogenic lesions in infants of epileptic mothers. Several lines of evidence indicate that PH may exert its action through the modification of phosphorylation patterns of neuronal polypeptides. We have studied the effects of PH on the phosphorylation of cytoskeletal proteins, because this could be related to the structural modifications induced by PH administration. The dendritic pattern of deep layers of the somatosensory cortex is clearly modified by PH but not the cell number, indicating that the drug disturbs the architecture of the neurons examined. In fact, the pattern of phosphorylation in cytoskeletal extracts of brains of 30-day-old rats is changed by PH. In vitro labeling experiments show decrease in the [32P] level of a 43-kDa polypeptide, whereas 38- and 120-kDa polypeptides show increases in their [32P] contents. The 43-kDa polypeptide has been identified as actin by in vitro experiments using a novel approach to determine cytoskeletal polypeptide behavior. We conclude that PH affects the posttranslational phosphorylation of actin and other related cytoskeletal proteins and in this manner may alter the normal morphological layout of dendritic patterns in the somatosensory cortex.
dc.fuente.origenWOS
dc.identifier.eissn1097-4547
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99557
dc.identifier.wosidWOS:A1987L306500012
dc.issue.numero3
dc.language.isoen
dc.pagina.final472
dc.pagina.inicio466
dc.revistaJournal of neuroscience research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEFFECT OF PHENYTOIN ON CYTOSKELETAL PROTEIN-PHOSPHORYLATION AND NEURONAL STRUCTURE IN THE RAT SENSORY CORTEX
dc.typeartículo
dc.volumen18
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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