Etoposide induces apoptosis and upregulation of TACE/ADAM17 and ADAM10 in an <i>in vitro</i> male germ cell line model

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dc.contributor.authorLizama, Carlos
dc.contributor.authorLudwig, Andreas
dc.contributor.authorMoreno, Ricardo D.
dc.date.accessioned2025-01-21T00:03:40Z
dc.date.available2025-01-21T00:03:40Z
dc.date.issued2011
dc.description.abstractEtoposide is a widely used anticancer drug in the treatment of different tumors. Etoposide is known to activate a wide range of intracellular signals, which may in turn induce cellular responses other than apoptosis. ADAM10 and TACE/ADAM17 belong to a family of transmembrane extracellular metalloproteinases involved in paracrine/juxtacrine regulation of many signaling pathways. The aim of this work was to evaluate if etoposide induces upregulation of ADAM10 or TACE/ADAM17 in two cell lines (GC-1 and GC-2) derived from male germ cells. Results showed that etoposide induced apoptosis in a dose-response manner in both GC-1 and GC-2 cells. Apoptosis started to increase 6 h after etoposide addition in GC-2 cells, whereas the same was observed 18 h after addition to the GC-1 cells. Protein and mRNA levels of ADAM10 and TACE/ADAM17 increased 18.h after etoposide was removed from the GC-1 cells. In GC-2 cells, the protein levels of both proteins increased 12 h after etoposide was removed. ADAM] 0 mRNA increased after 3 h and then steadily decreased up to 12 h after removal, whereas TACE/ADAM17 mRNA decreased after etoposide removal. Finally, apoptosis was prevented in GC-1 and GC-2 cells by the addition of pharmacological inhibitors of ADAM10 and TACE/ADAM17 to the culture medium of etoposide-treated cells. Our results show for the first time that etoposide upregulates ADAM10 and TACE/ADAM17 mRNA and protein levels. In addition, we also show that ADAM] 0 and TACE/ADAM17 have a role in etoposide-induced apoptosis. (c) 2010 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.bbamcr.2010.08.003
dc.identifier.eissn1879-2596
dc.identifier.issn0167-4889
dc.identifier.urihttps://doi.org/10.1016/j.bbamcr.2010.08.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95474
dc.identifier.wosidWOS:000286867800012
dc.issue.numero1
dc.language.isoen
dc.pagina.final128
dc.pagina.inicio120
dc.revistaBiochimica et biophysica acta-molecular cell research
dc.rightsacceso restringido
dc.subjectApoptosis
dc.subjectTestis
dc.subjectSpermatogenesis
dc.subjectEtoposide
dc.subjectTACE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEtoposide induces apoptosis and upregulation of TACE/ADAM17 and ADAM10 in an <i>in vitro</i> male germ cell line model
dc.typeartículo
dc.volumen1813
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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