Determination of a natural (17β-estradiol) and a synthetic (17α-ethinylestradiol) hormones in pharmaceutical formulations and urine by adsorptive stripping voltammetry

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dc.contributor.authorJose Trivino, Juan
dc.contributor.authorGomez, Marisol
dc.contributor.authorValenzuela, Javiera
dc.contributor.authorVera, Ariesny
dc.contributor.authorArancibia, Veronica
dc.date.accessioned2025-01-23T21:11:35Z
dc.date.available2025-01-23T21:11:35Z
dc.date.issued2019
dc.description.abstractAn efficient, fast and sensitive method for the determination of 17 beta-estradiol, (E2) and 17 alpha-ethinylestradiol (EE2) in pharmaceutical formulations and in urine was developed and validated using a hanging mercury drop electrode (HMDE), screen printed carbon electrodes (SPCE), and screen printed carbon nanotube electrodes (SPCNTE). Both analyzes are adsorbed on the working electrodes. To obtain sensitive and selective methods, the effects of various parameters such as pH, adsorption potential, and time (E-ads, t(ads)) were optimized. The optimum experimental conditions chosen for the two analyzes were pH: 10.0; E-ads: - 0.60 and t(ads): 30 s, when HMDE was used. Under these conditions, one reduction signal was found at -1.31 V for E2 and two reduction signals at - 0.23 V and -1.20 V for EE2. The detection limits (DLs) were found to be 0.3 mu g L-1 for E2, 14.8 mu g L-1 for EE2 at - 0.23 V, and 9.7 mu g L-1 for EE2 at -1.20 V. On the other hand, in screen printed electrodes E2 and EE2 present oxidation of the phenolic hydroxyl groups at 0.30, 0.31, 0.32, and 0.33 V (pH:10) with DLs of 242, 277; 182, and 191 mu g L-1 for SPCE and SPCNTE, respectively. The method was successfully applied to the determination of these analyzes in Primaquin (R) (E2), Gynera (R) (EE2), spiked urine (with EE2), and urine samples of women who used Tinelle (R) (EE2) as contraceptive drug.
dc.description.funderFondecyt
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.snb.2019.126728
dc.identifier.eissn0925-4005
dc.identifier.urihttps://doi.org/10.1016/j.snb.2019.126728
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100911
dc.identifier.wosidWOS:000478562700031
dc.language.isoen
dc.revistaSensors and actuators b-chemical
dc.rightsacceso restringido
dc.subject17 beta-estradiol (E2)
dc.subject17 alpha-ethinylestradiol (EE2)
dc.subjectPharmaceutical formulations
dc.subjectHuman urine
dc.subjectAdsorptive stripping voltammetry
dc.subject.ods03 Good Health and Well-being
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleDetermination of a natural (17β-estradiol) and a synthetic (17α-ethinylestradiol) hormones in pharmaceutical formulations and urine by adsorptive stripping voltammetry
dc.typeartículo
dc.volumen297
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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