DIAGNOSIS OF TYPHOID-FEVER BY 2 SEROLOGIC METHODS - ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF ANTILIPOPOLYSACCHARIDE OF SALMONELLA-TYPHI ANTIBODIES AND WIDAL TEST

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dc.contributor.authorQUIROGA, T
dc.contributor.authorGOYCOOLEA, M
dc.contributor.authorTAGLE, R
dc.contributor.authorGONZALEZ, F
dc.contributor.authorRODRIGUEZ, L
dc.contributor.authorVILLARROEL, L
dc.date.accessioned2025-01-23T19:22:01Z
dc.date.available2025-01-23T19:22:01Z
dc.date.issued1992
dc.description.abstractSerum samples from 85 patients with proven typhoid fever, 11 patients with p-typhoidal fever, 101 patients with febrile non-typhoidal, and 130 healthy subjects were tested for immunoglobulin G (IgG), IgA, and IgM antilipopolysaccharide (LPS) of Salmonella typhi antibodies by enzyme-linked immunosorbent assay (ELISA) and Widal test. The levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups; we selected various combinations between the three classes of immunoglobulin to obtain the best combination of sensitivity and specificity. The sum of the absorbance values obtained from the ELISA assay for IgG + IgA + IgM (SIGMAlgs) was the best choice for diagnostic utility for typhoid fever. We selected a positive test at a decision level of SIGMAlgs greater-than-or-equal-to 1.2 with a sensitivity of 94% and a specificity of 92% with a frequency of false negative of 5.9%. The frequency of false positives for healthy controls was 7.7% and, for the febrile nontyphoidal group, it was 7.9%. We also compared receiver (or relative) operating characteristic (ROC) curves for the diagnostic usefulness of the ELISA with that of the Widal test, whose merits and limitations, especially in endemic regions, are discussed. The ELISA assay was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.
dc.fuente.origenWOS
dc.identifier.issn0732-8893
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98935
dc.identifier.wosidWOS:A1992KG34200002
dc.issue.numero8
dc.language.isoen
dc.pagina.final656
dc.pagina.inicio651
dc.revistaDiagnostic microbiology and infectious disease
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDIAGNOSIS OF TYPHOID-FEVER BY 2 SEROLOGIC METHODS - ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF ANTILIPOPOLYSACCHARIDE OF SALMONELLA-TYPHI ANTIBODIES AND WIDAL TEST
dc.typeartículo
dc.volumen15
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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