BDNF/TrkB signaling endosomes in axons coordinate CREB/mTOR activation and protein synthesis in the cell body to induce dendritic growth in cortical neurons

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dc.contributor.authorMoya-Alvarado, Guillermo
dc.contributor.authorTiburcio-Felix, Reynaldo
dc.contributor.authorIbanez, Maria Raquel
dc.contributor.authorAguirre-Soto, Alejandro A.
dc.contributor.authorGuerra, Miguel, V
dc.contributor.authorWu, Chengbiao
dc.contributor.authorMobley, William C.
dc.contributor.authorPerlson, Eran
dc.contributor.authorBronfman, Francisca C.
dc.date.accessioned2025-01-20T20:16:44Z
dc.date.available2025-01-20T20:16:44Z
dc.date.issued2023
dc.description.abstractBrain-derived neurotrophic factor (BDNF) and its receptors tropomyosin kinase receptor B (TrkB) and the p75 neurotrophin receptor (p75) are the primary regulators of dendritic growth in the CNS. After being bound by BDNF, TrkB and p75 are endocytosed into endosomes and continue signaling within the cell soma, dendrites, and axons. We studied the functional role of BDNF axonal signaling in cortical neurons derived from different transgenic mice using compartmentalized cultures in microfluidic devices. We found that axonal BDNF increased dendritic growth from the neuronal cell body in a cAMP response element-binding protein (CREB)-dependent manner. These effects were dependent on axonal TrkB but not p75 activity. Dynein-dependent BDNF-TrkB-containing endosome transport was required for long-distance induction of dendritic growth. Axonal signaling endosomes increased CREB and mTOR kinase activity in the cell body, and this increase in the activity of both proteins was required for general protein translation and the expression of Arc, a plasticity-associated gene, indicating a role for BDNF-TrkB axonal signaling endosomes in coordinating the transcription and translation of genes whose products contribute to learning and memory regulation.
dc.fuente.origenWOS
dc.identifier.doi10.7554/eLife.77455.sa2
dc.identifier.issn2050-084X
dc.identifier.urihttps://doi.org/10.7554/eLife.77455
dc.identifier.urihttps://doi.org/10.7554/eLife.77455.sa0
dc.identifier.urihttps://doi.org/10.7554/eLife.77455.sa1
dc.identifier.urihttps://doi.org/10.7554/eLife.77455.sa2
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92350
dc.identifier.wosidWOS:000945965600001
dc.language.isoen
dc.revistaElife
dc.rightsacceso restringido
dc.subjectBDNF
dc.subjectTrkB
dc.subjectaxonal signaling
dc.subjectsignaling endosome
dc.subjectDynein
dc.subjectdendritic arborization
dc.subjectmTOR
dc.subjectCREB
dc.subjectMouse
dc.subjectRat
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBDNF/TrkB signaling endosomes in axons coordinate CREB/mTOR activation and protein synthesis in the cell body to induce dendritic growth in cortical neurons
dc.typeartículo
dc.volumen12
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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