Optimization of the Light-On system in a lentiviral platform to a light-controlled expression of genes in neurons

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dc.contributor.authorZarate, Rafaella, V
dc.contributor.authorArancibia, Duxan
dc.contributor.authorFernandez, Anllely
dc.contributor.authorSignorelli, Janetti R.
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorEstela Andres, Maria
dc.contributor.authorZamorano, Pedro
dc.date.accessioned2025-01-20T23:51:12Z
dc.date.available2025-01-20T23:51:12Z
dc.date.issued2021
dc.description.abstractBackground: Molecular brain therapies require the development of molecular switches to control gene expression in a limited and regulated manner in time and space. Light-switchable gene systems allow precise control of gene expression with an enhanced spatio-temporal resolution compared to chemical inducers. In this work, we adapted the existing light-switchable Light-On system into a lentiviral platform, which consists of two modules: (i) one for the expression of the blue light-switchable transactivator GAVPO and (ii) a second module containing an inducible-UAS promoter (UAS) modulated by a light-activated GAVPO.
dc.description.abstractResults: In the HEK293-T cell line transfected with this lentiviral plasmids system, the expression of the reporter mCherry increased between 4 to 5 fold after light induction. A time expression analysis after light induction during 24 h revealed that mRNA levels continuously increased up to 9 h, while protein levels increased throughout the experiment. Finally, transduction of cultured rat hippocampal neurons with this dual Light-On lentiviral system showed that CDNF, a potential therapeutic trophic factor, was induced only in cells exposed to blue light.
dc.description.abstractConclusions: In conclusion, the optimized lentiviral platform of the Light-On system provides an efficient way to control gene expression in neurons, suggesting that this platform could potentially be used in biomedical and neuroscience research, and eventually in brain therapies for neurodegenerative diseases. (C) 2021 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.ejbt.2021.03.006
dc.identifier.issn0717-3458
dc.identifier.urihttps://doi.org/10.1016/j.ejbt.2021.03.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94789
dc.identifier.wosidWOS:000652394100006
dc.language.isoen
dc.pagina.final57
dc.pagina.inicio50
dc.revistaElectronic journal of biotechnology
dc.rightsacceso restringido
dc.subjectGAVPO
dc.subjectGene expression in neurons
dc.subjectGene-regulated expression
dc.subjectLentiviral platform
dc.subjectLentivirus
dc.subjectLight-controlled expression
dc.subjectLight-On system
dc.subjectMolecular brain therapies
dc.subjectOptogenetic
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleOptimization of the Light-On system in a lentiviral platform to a light-controlled expression of genes in neurons
dc.typeartículo
dc.volumen51
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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