AXONS SPROUT AND MICROTUBULES INCREASE AFTER LOCAL INHIBITION OF RNA-SYNTHESIS, AND MICROTUBULES DECREASE AFTER INHIBITION OF PROTEIN-SYNTHESIS - A MORPHOMETRIC STUDY OF RAT SURAL NERVES

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dc.contributor.authorBUSTOS, J
dc.contributor.authorVIAL, JD
dc.contributor.authorFAUNDEZ, V
dc.contributor.authorALVAREZ, J
dc.date.accessioned2025-01-23T19:22:29Z
dc.date.available2025-01-23T19:22:29Z
dc.date.issued1991
dc.description.abstractDrugs that inhibit RNA or protein synthesis are known to affect some functional properties of axons. In this context, we studied the ultrastructural effects of actinomycin-D, an inhibitor of RNA synthesis, and cycloheximide and emetine, inhibitors of protein synthesis, in rat sural nerves. A silicone sleeve (4 mm long) loaded with drug was placed around the nerves and left for about a week. The ultrastructural alterations of axons and Schwann cells progressed over this period. After cycloheximide and emetine, the cytoplasm of Schwann cells was enlarged and the rough endoplasmic reticulum was prominent. After actinomycin-D, the Schwann cells reached the stage of lysis. Nonmedullated were more affected than myelinated axons. After cycloheximide and emetine, the axoplasmic matrix decreased substantially but reversibly. Microtubules of nonmedullated fibres decreased by about 50%. Actinomycin-D determined sprouting of axons and a rise of axonal microtubules; in nonmedullated axons, the normal inverse correlation between microtubular density and calibre gave way to a high and constant density for all axonal sizes. A few millimetres proximal and distal to the sleeve, the nerve tissue and the axonal microtubular content were close to normal, i.e. the effects of drugs were local. Present results suggest that the local turnover of amino acids in the axon is necessary to maintain the integrity of microtubule and neurofilament proteins. We propose that the Schwann cell down-regulates the axonal cytomatrix.
dc.fuente.origenWOS
dc.identifier.issn0953-816X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99044
dc.identifier.wosidWOS:A1991GP60600007
dc.issue.numero11
dc.language.isoen
dc.pagina.final1133
dc.pagina.inicio1123
dc.revistaEuropean journal of neuroscience
dc.rightsacceso restringido
dc.subjectCYTOSKELETON
dc.subjectCYCLOHEXIMIDE
dc.subjectEMETINE
dc.subjectTRANSCRIPTION
dc.subjectACTINOMYCIN-D
dc.subjectTAXOL
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAXONS SPROUT AND MICROTUBULES INCREASE AFTER LOCAL INHIBITION OF RNA-SYNTHESIS, AND MICROTUBULES DECREASE AFTER INHIBITION OF PROTEIN-SYNTHESIS - A MORPHOMETRIC STUDY OF RAT SURAL NERVES
dc.typeartículo
dc.volumen3
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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