Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

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dc.contributor.authorTorres, Daniela
dc.contributor.authorMongiardini, Elias
dc.contributor.authorDonadio, Florencia
dc.contributor.authorDonoso, Raul
dc.contributor.authorRecabarren-Gajardo, Gonzalo
dc.contributor.authorGualpa, Jose
dc.contributor.authorSpaepen, Stijn
dc.contributor.authorDefez, Roberto
dc.contributor.authorLopez, Gaston
dc.contributor.authorBianco, Carmen
dc.contributor.authorCassan, Fabricio
dc.date.accessioned2025-01-20T22:19:23Z
dc.date.available2025-01-20T22:19:23Z
dc.date.issued2021
dc.description.abstractBradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physio-logical features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits a and b) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
dc.description.abstract(c) 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.resmic.2021.103814
dc.identifier.eissn1769-7123
dc.identifier.issn0923-2508
dc.identifier.urihttps://doi.org/10.1016/j.resmic.2021.103814
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94595
dc.identifier.wosidWOS:000661425600006
dc.issue.numero3
dc.language.isoen
dc.revistaResearch in microbiology
dc.rightsacceso restringido
dc.subjectB
dc.subjectjaponicum
dc.subjectIndole-3-acetic acid
dc.subject3-Phenylpropionate dioxygenase
dc.subject3-Indoleacetic acid 2
dc.subject3-Oxide
dc.subject2-(2-Hydroperoxy-3-hydroxyindolin-3-yl) acetic acid
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleMolecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
dc.typeartículo
dc.volumen172
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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