Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
| dc.contributor.author | Jaimovich, E | |
| dc.contributor.author | Espinosa, A | |
| dc.date.accessioned | 2025-01-21T01:08:24Z | |
| dc.date.available | 2025-01-21T01:08:24Z | |
| dc.date.issued | 2004 | |
| dc.description.abstract | The study of fluorescent calcium signals from cultured rat myotubes has provided interesting results in the past few years. Both K+ depolarization and tetanic electrical stimulation were shown to produce slow Ca2+ signals, unrelated to contraction and associated to regulation of gene expression in cultured rat myotubes. We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The different intracellular Ca2+ patterns produced by electrical stimulation, testosterone, IGF-I and insulin, may help to understand the role of intracellular calcium kinetics in the regulation of gene expression by various stimuli in skeletal muscle cells. | |
| dc.fuente.origen | WOS | |
| dc.identifier.eissn | 0717-6287 | |
| dc.identifier.issn | 0716-9760 | |
| dc.identifier.uri | https://repositorio.uc.cl/handle/11534/96463 | |
| dc.identifier.wosid | WOS:000226853600018 | |
| dc.issue.numero | 4 | |
| dc.language.iso | en | |
| dc.pagina.final | 633 | |
| dc.pagina.inicio | 625 | |
| dc.revista | Biological research | |
| dc.rights | acceso restringido | |
| dc.subject | skeletal | |
| dc.subject | transcription factors | |
| dc.subject | insulin | |
| dc.subject | IGF-I | |
| dc.subject | testosterone | |
| dc.subject.ods | 03 Good Health and Well-being | |
| dc.subject.odspa | 03 Salud y bienestar | |
| dc.title | Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells | |
| dc.type | artículo | |
| dc.volumen | 37 | |
| sipa.index | WOS | |
| sipa.trazabilidad | WOS;2025-01-12 |