Wnt-5a/Ca2+pathway modulates endogenous current and oocyte structure of Xenopus laevis

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dc.contributor.authorParodi, Jorge
dc.contributor.authorMira, Rodrigo G.
dc.contributor.authorMartinez-Torres, Ataulfo
dc.contributor.authorInestrosa, Nibaldo C.
dc.date.accessioned2025-01-20T16:09:58Z
dc.date.available2025-01-20T16:09:58Z
dc.date.issued2024
dc.description.abstractWnt signaling plays an essential role in cellular processes like development, maturation, and function maintenance. Xenopus laevis oocytes are a suitable model to study not only the development but also the function of different receptors expressed in their membranes, like those receptors expressed in the central nervous system (CNS) including Frizzled 7. Here, using frog oocytes and recordings of endogenous membrane currents in a two- electrode path configuration along with morphological observations, we evaluated the role of the non-canonical Wnt-5a ligand in oocytes. We found that acute application of Wnt-5a generated changes in endogenous calcium- dependent currents, entry oscillatory current, the membrane's outward current, and induced membrane depolarization. The incubation of oocytes with Wnt-5a caused a reduction of the membrane potential, potassium outward current, and protected the ATP current in the epithelium/theca removed (ETR) model. The oocytes exposed to Wnt-5a showed increased viability and an increase in the percentage of the germinal vesicle breakdown (GVBD), at a higher level than the control with progesterone. Altogether, our results suggest that Wnt-5a modulates different aspects of oocyte structure and generates calcium-dependent endogenous current alteration and GVDB process with a change in membrane potential at different concentrations and times of the exposition. These results help to understand the cellular effect of Wnt-5a and present the use of Xenopus oocytes to explore the mechanism that could impact the activation of Wnt signaling.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.bbrc.2024.150507
dc.identifier.eissn1090-2104
dc.identifier.issn0006-291X
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2024.150507
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90187
dc.identifier.wosidWOS:001297893100001
dc.language.isoen
dc.revistaBiochemical and biophysical research communications
dc.rightsacceso restringido
dc.subjectNon-canonical Wnt-5a signaling
dc.subjectGerminal vesicle
dc.subjectOocytes
dc.subjectXenopus laevis
dc.subjectCa2+endogenous current
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleWnt-5a/Ca2+pathway modulates endogenous current and oocyte structure of Xenopus laevis
dc.typeartículo
dc.volumen738
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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