Signal pathway profiling of prostate cancer using reverse phase protein arrays

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dc.contributor.authorGrubb, RL
dc.contributor.authorCalvert, VS
dc.contributor.authorWulkuhle, JD
dc.contributor.authorPaweletz, CP
dc.contributor.authorLinehan, WM
dc.contributor.authorPhillips, JL
dc.contributor.authorChuaqui, R
dc.contributor.authorValasco, A
dc.contributor.authorGillespie, J
dc.contributor.authorEmmert-Buck, M
dc.contributor.authorLiotta, LA
dc.contributor.authorPetricoin, EF
dc.date.accessioned2025-01-21T01:08:44Z
dc.date.available2025-01-21T01:08:44Z
dc.date.issued2003
dc.description.abstractReverse phase protein arrays represent a new proteomics microarray technology with which to study the fluctuating state of the proteome in minute quantities of cells. The activation status of cell signaling pathways controls cellular fate and deregulation of these pathways underpins carcinogenesis. Changes in pathway activation that occur between early stage prostatic epithelial lesions, prostatic stroma and the extracellular matrix can be analyzed by obtaining pure populations of cell types by laser capture microdissection (LCM) and analyzing the relative states of several key phosphorylation points within the cellular circuitry. We have applied reverse phase protein array technology to analyze the status of key points in cell signaling involved in pro-survival, mitogenic, apoptotic and growth regulation pathways in the progression from normal prostate epithelium to invasive prostate cancer. Using multiplexed reverse phase protein arrays coupled with LCM, the states of signaling changes during disease progression from prostate cancer study sets were analyzed. Focused analysis of phosphospecific endpoints revealed changes in cellular signaling events through disease progression and, between patients. We have used a new protein array technology to study specific molecular pathways believed to be important in cell survival and progression from normal epithelium to invasive carcinoma directly from human tissue specimens. With the advent of molecular targeted therapeutics, the identification, characterization and monitoring of the signaling events within actual human biopsies will be critical for patient-tailored therapy.
dc.fuente.origenWOS
dc.identifier.doi10.1002/pmic.200300598
dc.identifier.issn1615-9853
dc.identifier.urihttps://doi.org/10.1002/pmic.200300598
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96503
dc.identifier.wosidWOS:000186582500010
dc.issue.numero11
dc.language.isoen
dc.pagina.final2146
dc.pagina.inicio2142
dc.revistaProteomics
dc.rightsacceso restringido
dc.subjectprostate cancer
dc.subjectprotein microarrays
dc.subjectsignal transduction profiling
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSignal pathway profiling of prostate cancer using reverse phase protein arrays
dc.typeartículo
dc.volumen3
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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