Oral infectivity through carnivorism in murine model of <i>Trypanosoma cruzi</i> infection

Simple item page
dc.contributor.authorTorres, Victor
dc.contributor.authorContreras, Victor
dc.contributor.authorGutierrez, Bessy
dc.contributor.authorSan Francisco, Juan
dc.contributor.authorCatalan, Alejandro
dc.contributor.authorVega, Jose Luis
dc.contributor.authorMoon, Kyung-Mee
dc.contributor.authorFoster, Leonard J.
dc.contributor.authorde Almeida, Rafael F.
dc.contributor.authorKalergis, Alexis M.
dc.contributor.authorGonzalez, Jorge
dc.date.accessioned2025-01-20T17:07:56Z
dc.date.available2025-01-20T17:07:56Z
dc.date.issued2024
dc.description.abstractIntroduction Oral transmission of T. cruzi is probably the most frequent transmission mechanism in wild animals. This observation led to the hypothesis that consuming raw or undercooked meat from animals infected with T. cruzi may be responsible for transmitting the infection. Therefore, the general objective of this study was to investigate host-pathogen interactions between the parasite and gastric mucosa and the role of meat consumption from infected animals in the oral transmission of T. cruzi. Methods Cell infectivity assays were performed on AGS cells in the presence or absence of mucin, and the roles of pepsin and acidic pH were determined. Moreover, groups of five female Balb/c mice were fed with muscle tissue obtained from mice in the acute phase of infection by the clone H510 C8C3 hvir of T. cruzi, and the infection of the fed mice was monitored by a parasitemia curve. Similarly, we assessed the infective capacity of T. cruzi trypomastigotes and amastigotes by infecting groups of five mice Balb/c females, which were infected orally using a nasogastric probe, and the infection was monitored by a parasitemia curve. Finally, different trypomastigote and amastigote inoculums were used to determine their infective capacities. Adhesion assays of T. cruzi proteins to AGS stomach cells were performed, and the adhered proteins were detected by western blotting using monoclonal or polyclonal antibodies and by LC-MS/MS and bioinformatics analysis. Results Trypomastigote migration in the presence of mucin was reduced by approximately 30%, whereas in the presence of mucin and pepsin at pH 3.5, only a small proportion of parasites were able to migrate (similar to 6%). Similarly, the ability of TCTs to infect AGS cells in the presence of mucin is reduced by approximately 20%. In all cases, 60-100% of the animals were fed meat from mice infected in the acute phase or infected with trypomastigotes or amastigotes developed high parasitemia, and 80% died around day 40 post-infection. The adhesion assay showed that cruzipain is a molecule of trypomastigotes and amastigotes that binds to AGS cells. LC-MS/MS and bioinformatics analysis, also confirmed that transialidase, cysteine proteinases, and gp63 may be involved in TCTs attachment or invasion of human stomach cells because they can potentially interact with different proteins in the human stomach mucosa. In addition, several human gastric mucins have cysteine protease cleavage sites. Discussion Then, under our experimental conditions, consuming meat from infected animals in the acute phase allows the T. cruzi infection. Similarly, trypomastigotes and amastigotes could infect mice when administered orally, whereas cysteinyl proteinases and trans-sialidase appear to be relevant molecules in this infective process.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fcimb.2024.1297099
dc.identifier.issn2235-2988
dc.identifier.urihttps://doi.org/10.3389/fcimb.2024.1297099
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90909
dc.identifier.wosidWOS:001185195100001
dc.language.isoen
dc.revistaFrontiers in cellular and infection microbiology
dc.rightsacceso restringido
dc.subjectTrypanosoma cruzi
dc.subjectoral infection
dc.subjectcruzipain
dc.subjecttrans-sialidase
dc.subjecthost-pathogen interaction
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleOral infectivity through carnivorism in murine model of <i>Trypanosoma cruzi</i> infection
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Oral.pdf
Size:
4.79 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas