SNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics

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dc.contributor.authorCabrera-Reyes, Fernanda
dc.contributor.authorContreras-Palacios, Teemly
dc.contributor.authorUlloa, Romina
dc.contributor.authorJara-Wilde, Jorge
dc.contributor.authorCaballero, Mia
dc.contributor.authorQuiroga, Clara
dc.contributor.authorFeijoo, Carmen G.
dc.contributor.authorDiaz-Munoz, Jheimmy
dc.contributor.authorYuseff, Maria-Isabel
dc.date.accessioned2025-01-20T16:05:05Z
dc.date.available2025-01-20T16:05:05Z
dc.date.issued2024
dc.description.abstractB cells rapidly adapt their endocytic pathway to promote the uptake and processing of extracellular antigens recognized through the B-cell receptor (BCR). The mechanisms coupling changes in endomembrane trafficking to the capacity of B cells to screen for antigens within lymphoid tissues remain unaddressed. We investigated the role of SNX5, a member of the sorting nexin family, which interacts with endocytic membranes to regulate vesicular trafficking and macropinocytosis. Our results show that in steady state, B cells form SNX5-rich protrusions at the plasma membrane, which dissipate upon interaction with soluble antigens, whereas B cells activated with immobilized antigens accumulate SNX5 at the immune synapse where it regulates actin-dependent spreading responses. B cells silenced for SNX5 exhibit enlarged lysosomes, which are not recruited to the synaptic membrane, decreasing their capacity to extract immobilized antigens. Overall, our findings reveal that SNX5 is critical for actin-dependent plasma membrane remodeling in B cells involved in antigen screening and immune synapse formation, as well as endolysosomal trafficking required to promote antigen extraction and presentation.
dc.fuente.origenWOS
dc.identifier.doi10.26508/lsa.202402917
dc.identifier.eissn2575-1077
dc.identifier.urihttps://doi.org/10.26508/lsa.202402917
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89865
dc.identifier.wosidWOS:001343195700001
dc.issue.numero1
dc.language.isoen
dc.revistaLife science alliance
dc.rightsacceso restringido
dc.titleSNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics
dc.typeartículo
dc.volumen8
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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