G<sub>2</sub> repair and chromosomal damage in lymphocytes from workers occupationally exposed to low-level ionizing radiation

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dc.contributor.authorPincheira, J
dc.contributor.authorLópez, I
dc.contributor.authorSanhueza, S
dc.contributor.authorFerruz, P
dc.contributor.authorNavarrete, MH
dc.contributor.authorSantos, MJ
dc.contributor.authorLópez-Sáez, JF
dc.date.accessioned2025-01-21T01:32:14Z
dc.date.available2025-01-21T01:32:14Z
dc.date.issued1999
dc.description.abstractThe effect of the G(2) repair of chromosomal damage in lymphocytes from workers exposed to low levels of X- or gamma-rays was evaluated, Samples of peripheral blood were collected from 15 radiation workers, 20 subjects working in radiodiagnostics, and 30 healthy control donors.
dc.description.abstractChromosomal aberrations (CA) were evaluated by scoring the presence of chromatid and isochromatid breaks, dicentric and ring chromosomes in lymphocytes with/without 5mM caffeine plus 3mM-aminobenzamide (3-AB) treatment during G(2).
dc.description.abstractOur results showed that the mean value of basal aberrations in lymphocytes from exposed workers was higher than in control cells (p < 0.001). The chromosomal damage in G(2), detected with caffeine plus 3-AB treatment was higher than the basal damage (untreated conditions), both in control and exposed populations (p< 0.05). In the exposed workers group, the mean value of chromosomal abnormalities in G(2) was higher than in the control (p< 0.0001).
dc.description.abstractNo correlation was found between the frequency of chromosome type of aberrations (basal or in G(2)), and the absorbed dose. Nevertheless, significant correlation coefficients (p< 0.05) between absorbed dose and basal aberrations yield (r = 0.430) or in G(2) (r = 0.448) were detected when chromatid breaks were included in the total aberrations yield. Under this latter condition no significant effect of age, years of employment or smoking habit on the chromosomal aberrations yield was detected. However, analysis of the relationship between basal aberrations yield and the efficiency of G(2) repair mechanisms, defined as the percentage of chromosomal lesions repaired in G(2), showed a significant correlation coefficient (r = -0.802; p< 0.001).
dc.description.abstractThese results suggest that in addition to the absorbed dose, the individual G(2) repair efficiency may be another important factor. affecting the chromosomal aberrations yield detected in workers exposed to low-level ionizing radiation.
dc.fuente.origenWOS
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97230
dc.identifier.wosidWOS:000087492400006
dc.issue.numero4
dc.language.isoen
dc.pagina.final306
dc.pagina.inicio297
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectchromosome aberrations ionizing radiation
dc.subjectG(2) repair
dc.subjecthuman lymphocytes
dc.subjectoccupational exposure
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleG<sub>2</sub> repair and chromosomal damage in lymphocytes from workers occupationally exposed to low-level ionizing radiation
dc.typeartículo
dc.volumen32
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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