Ceftazidime/avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean clinical isolates of non-carbapenemase producing <i>Pseudomonas aeruginosa</i>

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dc.contributor.authorSoto, Katherine D.
dc.contributor.authorAlcalde-Rico, Manuel
dc.contributor.authorUgalde, Juan A.
dc.contributor.authorOlivares-Pacheco, Jorge
dc.contributor.authorQuiroz, Valeria
dc.contributor.authorBrito, Barbara
dc.contributor.authorRivas, Lina M.
dc.contributor.authorMunita, Jose M.
dc.contributor.authorGarcia, Patricia C.
dc.contributor.authorWozniak, Aniela
dc.date.accessioned2025-01-20T16:15:30Z
dc.date.available2025-01-20T16:15:30Z
dc.date.issued2024
dc.description.abstractIntroduction Ceftazidime/avibactam (CZA) is indicated against multidrug-resistant Pseudomonas aeruginosa, particularly those that are carbapenem resistant. CZA resistance in P. aeruginosa producing PER, a class A extended-spectrum beta-lactamase, has been well documented in vitro. However, data regarding clinical isolates are scarce. Our aim was to analyze the contribution of PER to CZA resistance in non-carbapenemase-producing P. aeruginosa clinical isolates that were ceftazidime and/or carbapenem non-susceptible. Methods Antimicrobial susceptibility was determined through agar dilution and broth microdilution, while bla(PER) gene was screened through PCR. All PER-positive isolates and five PER-negative isolates were analyzed through Whole Genome Sequencing. The mutational resistome associated to CZA resistance was determined through sequence analysis of genes coding for PBPs 1b, 3 and 4, MexAB-OprM regulators MexZ, MexR, NalC and NalD, AmpC regulators AmpD and AmpR, and OprD porin. Loss of bla(PER-3) gene was induced in a PER-positive isolate by successive passages at 43 degrees C without antibiotics. Results Twenty-six of 287 isolates studied (9.1%) were CZA-resistant. Thirteen of 26 CZA-resistant isolates (50%) carried bla(PER). One isolate carried bla(PER) but was CZA-susceptible. PER-producing isolates had significantly higher MICs for CZA, amikacin, gentamicin, ceftazidime, meropenem and ciprofloxacin than non-PER-producing isolates. All PER-producing isolates were ST309 and their bla(PER-3) gene was associated to ISCR1, an insertion sequence known to mobilize adjacent DNA. PER-negative isolates were classified as ST41, ST235 (two isolates), ST395 and ST253. PER-negative isolates carried genes for narrow-spectrum beta-lactamases and the mutational resistome showed that all isolates had one major alteration in at least one of the genes analyzed. Loss of bla(PER-3) gene restored susceptibility to CZA, ceftolozane/tazobactam and other beta-lactamsin the in vitro evolved isolate. Discussion PER-3-producing ST309 P. aeruginosa is a successful multidrug-resistant clone with bla(PER-3) gene implicated in resistance to CZA and other beta-lactams.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fcimb.2024.1410834
dc.identifier.issn2235-2988
dc.identifier.urihttps://doi.org/10.3389/fcimb.2024.1410834
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90498
dc.identifier.wosidWOS:001250656000001
dc.language.isoen
dc.revistaFrontiers in cellular and infection microbiology
dc.rightsacceso restringido
dc.subjectPseudomonas aeruginosa
dc.subjectblaPER-3 gene
dc.subjectPER-3 extended-spectrum beta-lactamase
dc.subjectceftazidime/avibactam resistance
dc.subjectmutations conferring CZA resistance
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCeftazidime/avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean clinical isolates of non-carbapenemase producing <i>Pseudomonas aeruginosa</i>
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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