Rapid diagnosis of major variants of congenital epidermolysis bullosa using a monoclonal antibody against collagen type IV

dc.contributor.authorBolte, C
dc.contributor.authorGonzalez, S
dc.date.accessioned2025-01-21T01:34:20Z
dc.date.available2025-01-21T01:34:20Z
dc.date.issued1995
dc.description.abstractA simple and rapid procedure to diagnose major variants of congenital epidermolysis bullosa (EB) by means of immunohistochemical staining of collagen IV (CIV) in for malin-fixed and paraffin-embedded samples is described. Sixteen cases were stained with a monoclonal antibody (clone CIV 22) against collagen IV present in the lamina densa layer of the basement membrane. Electron microscopy was performed simultaneously. Twelve cases showed one of two staining patterns: an EB simplex pattern (seven cases) consisting of linear and continuous staining on the ''floor'' of the blister and an EB dystrophica pattern (five cases) showing a linear and discontinuous staining on the ''roof'' of the blister. In four cases, collagen IV staining was not reactive because of irreversible antigen loss due to inadequate fixation and/or embedding. In those cases with positive staining, an excellent correlation with electron microscopic diagnosis was achieved. This procedure allows a rapid diagnosis of major EB variants, especially where electron microscopy is not available, and also facilitates diagnosis by optimally orienting specimens for ultrastructural examination when available.
dc.fuente.origenWOS
dc.identifier.issn0193-1091
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97544
dc.identifier.wosidWOS:A1995TM83400009
dc.issue.numero6
dc.language.isoen
dc.pagina.final583
dc.pagina.inicio580
dc.revistaAmerican journal of dermatopathology
dc.rightsacceso restringido
dc.subjectepidermolysis bullosa simplex
dc.subjectepidermolysis bullosa dystrophica
dc.subjectcollagen IV
dc.subjectimmunohistochemistry
dc.subjectepidermolysis bullosa hereditaria
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRapid diagnosis of major variants of congenital epidermolysis bullosa using a monoclonal antibody against collagen type IV
dc.typeartículo
dc.volumen17
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files