Identificación de asociaciones clínico-patológicas e hipermetilación de genes supresores de tumores en cáncer gástrico difuso a través de análisis de hierarchical clustering

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dc.catalogadorpau
dc.contributor.authorZavala G., Luis
dc.contributor.authorLuengo J., Víctor
dc.contributor.authorOssandón C., Francisco
dc.contributor.authorRiquelme S., Erick
dc.contributor.authorBackhouse E., Claudia
dc.contributor.authorPalma V., Mariana
dc.contributor.authorArgandoña C., Jorge
dc.contributor.authorCumsille, Miguel Angel
dc.contributor.authorCorvalán R., Alejandro
dc.date.accessioned2024-06-09T02:37:29Z
dc.date.available2024-06-09T02:37:29Z
dc.date.issued2007
dc.description.abstractBackground: Methylation is an inactivation mechanism for tumor suppressor genes, that can have important clinical implications. Aim: To analyze the methylation status of 11 tumor suppressor genes in pathological samples of diffuse gastric cancer. Material and methods: Eighty three patients with diffuse gastric cancer with information about survival and infection with Epstein Barr virus, were studied. DNA was extracted from pathological slides and the methylation status of genes p14, p15, p16, APC, p73, FHIT, E-caderin, SEMA3B, BRCA-1, MINT-2 y MGMT, was studied using sodium bisulphite modification and polymerase chain reaction. Results were grouped according to the methylation index or Hierarchical clustering (TIGR MultiExperiment Viewer). Results: Three genes had a high frequency of methylation (FHIT, BRCA1, APC), four had an intermediate frequency (p15, MGMT, p14, MINT2) and four had a low frequency (p16, p73, E-cadherin, SEMA3B). The methylation index had no association with clinical or pathological features of tumors or patients survival. Hierarchical clustering generated two clusters. One grouped clinical and pathological features with FHIT, BRCA1, and APC and the other grouped the other eight genes and Epstein Barr virus infection. Two significant associations were found, between APC and survival and p16/p14 and Epstein Barr virus infection. Conclusions: Hierarchical clustering is a tool that identifies associations between clinical and pathological features of tumors and methylation of tumor suppressor genes.
dc.fechaingreso.objetodigital2024-06-09
dc.fuente.origenScopus
dc.identifier.eissn0717-6163
dc.identifier.pubmedid17369979
dc.identifier.scopusidSCOPUS_ID:33947178593
dc.identifier.urihttp://www.scielo.cl/pdf/rmc/v135n1/art03.pdf
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/86649
dc.language.isoes
dc.nota.accesocontenido completo
dc.pagina.final25
dc.pagina.inicio17
dc.revistaRevista Médica de Chile
dc.rightsacceso abierto
dc.subjectCluster analysis
dc.subjectGenes
dc.subjectStomach neoplasms
dc.subjectSupressor
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleIdentificación de asociaciones clínico-patológicas e hipermetilación de genes supresores de tumores en cáncer gástrico difuso a través de análisis de hierarchical clustering
dc.title.alternativeHierarchical clustering analysis to detect associations between clinical and pathological features of gastric tumors and hypermethylation of suppressor genes
dc.typeartículo
dc.volumen135
sipa.trazabilidadScopus;12-10-2021
sipa.trazabilidadORCID;2024-06-03
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