Functional interactions between sulphated polysaccharides and proacrosin: implications in sperm binding and digestion of zona pellucida

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dc.contributor.authorMoreno, RD
dc.contributor.authorHoshi, M
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:32:05Z
dc.date.available2025-01-21T01:32:05Z
dc.date.issued1999
dc.description.abstractAcrosin is a serine protease located within mammalian acrosome as inactive proacrosin. Sulphated polymers bind to proacrosin and acrosin, to a domain different from the active site. Upon binding, these polymers induce proacrosin activation and some of them, such as fucoidan, inhibit sperm binding to the zona pellucida. In this work we have studied the interaction of solubilised zona pellucida glycoproteins (ZPGs), heparin and ARTS (Acrosome Reaction Inducing Substance of Starfish) with boar and human acrosin. We have found that ARIS, solubilised ZPGs and fucoidan, but not heparin, inhibit the binding of the monoclonal antibody against human acrosin C5F10 to boar or human proacrosin. These results suggest that fucoidan, solubilised ZPGs and ARIS bind to a related domain on the proacrosin surface. Moreover, ARIS was able to induce human proacrosin activation. On the other hand, neither ARIS nor heparin from porcine intestinal mucosa or bovine lung induced hamster sperm acrosome reaction or sperm motility. Recent data showed that acrosin is involved in dispersal of the acrosomal matrix after acrosome reaction. Thus, the control of the ZPG glycan chains over proacrosin activation may regulate both sperm penetration rate and limited proteolysis of zona pellucida proteins.
dc.fuente.origenWOS
dc.identifier.eissn1469-8730
dc.identifier.issn0967-1994
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97202
dc.identifier.wosidWOS:000083066100002
dc.issue.numero2
dc.language.isoen
dc.pagina.final111
dc.pagina.inicio105
dc.revistaZygote
dc.rightsacceso restringido
dc.subjectacrosin
dc.subjectARIS
dc.subjectheparin
dc.subjectsecondary binding
dc.subjectzona pellucida
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFunctional interactions between sulphated polysaccharides and proacrosin: implications in sperm binding and digestion of zona pellucida
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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