Generation and analysis of expressed sequence tags from <i>Botrytis cinerea</i>

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dc.contributor.authorSilva, Evelyn
dc.contributor.authorValdes, Jorge
dc.contributor.authorHolmes, David
dc.contributor.authorShmaryahu, Amir
dc.contributor.authorValenzuela, Pablo D. T.
dc.date.accessioned2025-01-21T01:06:30Z
dc.date.available2025-01-21T01:06:30Z
dc.date.issued2006
dc.description.abstractBotrytis cinerea is a filamentous plant pathogen of a wide range of plant species. and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11.482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3.032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism. 12% for transport of metabolites. and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli. cell communication. cell homeostasis, and cell development. We carried Out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%. respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96168
dc.identifier.wosidWOS:000238917800018
dc.issue.numero2
dc.language.isoen
dc.pagina.final376
dc.pagina.inicio367
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectBotrytis cinerea
dc.subjectcDNA sequencing
dc.subjectfungal pathogenicity
dc.titleGeneration and analysis of expressed sequence tags from <i>Botrytis cinerea</i>
dc.typeartículo
dc.volumen39
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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