Regulation of hepatic connexins in cholestasis

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dc.contributor.authorGonzález, HE
dc.contributor.authorEugenín, EA
dc.contributor.authorGarcés, G
dc.contributor.authorSolís, N
dc.contributor.authorPizarro, M
dc.contributor.authorAccatino, L
dc.contributor.authorSáez, JC
dc.date.accessioned2025-01-21T01:30:24Z
dc.date.available2025-01-21T01:30:24Z
dc.date.issued2002
dc.description.abstractHepatocyte gap junction proteins, connexins (Cxs) 26 and 32, are downregulated during obstructive cholestasis (OC) and lipopolysaccharide hepatocellular cholestasis (LPS-HC). We investigated rat hepatic Cxs during ethynylestradiol hepatocellular cholestasis (EE-HC) and choledochocaval fistula (CCF) and compared them with OC and LPS-HC. Levels (immunoblotting) and cellular distribution (immunofluorescence) of Cx26, -32, and -43, as well as macrophage infiltration, were studied in livers of rats under each condition. Cx26 and -32 were reduced in LPS-HC, OC, and CCF. However, in EE-HC, Cx26 did not change and Cx32 was increased. Prominent inflammation occurred in LPS-HC, OC, and CCF, which was associated with increased levels of Cx43 in LPS-HC and OC but not CCF. No inflammation nor changes in Cx43 levels occurred during EE-HC. In cultured hepatocytes, dye coupling was reduced by tumor necrosis factor-alpha and interleukins-1beta and -6, whereas reduction induced by LPS required coculture with Kupffer cells. Thus hepatocyte gap junctions are downregulated in forms of cholestasis associated with inflammation, and reduced intercellular communication might be induced in part by proinflammatory mediators.
dc.fuente.origenWOS
dc.identifier.doi10.1152/ajpgi.00298.2001
dc.identifier.eissn1522-1547
dc.identifier.issn0193-1857
dc.identifier.urihttps://doi.org/10.1152/ajpgi.00298.2001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96742
dc.identifier.wosidWOS:000175620100010
dc.issue.numero6
dc.language.isoen
dc.pagina.finalG1001
dc.pagina.inicioG991
dc.revistaAmerican journal of physiology-gastrointestinal and liver physiology
dc.rightsacceso restringido
dc.subjectobstructive cholestasis
dc.subjecthepatocellular cholestasis
dc.subjectcytokines
dc.subjectmacrophages
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRegulation of hepatic connexins in cholestasis
dc.typeartículo
dc.volumen282
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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