Characterization of small RNAs in X. tropicalis gastrulae

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dc.catalogadoraba
dc.contributor.authorFaunes Quinteros, Fernando Emerson
dc.contributor.authorAlmonacid, L. I.
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorLarraín Correa, Juan Agustín
dc.date.accessioned2025-02-05T19:40:30Z
dc.date.available2025-02-05T19:40:30Z
dc.date.issued2012
dc.description.abstractHere, we report and characterize deep sequencing data and bioinformatics analysis of small RNAs from X. tropicalis gastrula. A total of 17,553,124 reads with perfect match to the genome derived from 2,616,053 unique sequences were identified. Seventy-seven percent of theses sequences were not found in previous reports from X. tropicalis oocytes and somatic tissues. Bioinformatics analyses indicate that a large fraction of the small RNAs are PIWI-interacting RNAs. Up to 23.9% of small RNAs mapped to transposable elements and 27% to genic regions. Most of the abundant transposon-derived small RNAs are found in oocyte and gastrula libraries, suggesting that transposons also need to be silenced during early embryonic development. Importantly, novel clusters of piRNAs whose expression is activated after zygotic transcription begins were identified in the genome of X. tropicalis. Additionally, miRNAs were also identified and many of them are not present in oocytes, suggesting that miRNA expression is stage-specific. To the best of our knowledge, this is the first high throughput data release and bioinformatics characterization of small RNAs during Xenopus early embryonic development. genesis 50:572–58, 2012. © 2012 Wiley Periodicals, Inc.
dc.fuente.origenSIPA
dc.identifier.doi10.1002/dvg.22027
dc.identifier.eissn1526-968X
dc.identifier.issn1526-954X
dc.identifier.scopusid2-s2.0-84863991130
dc.identifier.urihttps://doi.org/10.1002/dvg.22027
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102159
dc.identifier.wosidWOS:000306491100007
dc.information.autorucFacultad de Ciencias Biológicas; Faunes Quinteros, Fernando Emerson; S/I; 13517
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.information.autorucFacultad de Ciencias Biológicas; Larraín Correa, Juan Agustín; S/I; 90468
dc.issue.numero7
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final583
dc.pagina.inicio572
dc.revistaGenesis (New York, N.Y. : 2000)
dc.rightsacceso restringido
dc.subjectGastrula
dc.subjectXenopus
dc.subjectSmall RNAs
dc.subjectPiwi-interactingRNAs
dc.subjectTransposable elements
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCharacterization of small RNAs in X. tropicalis gastrulae
dc.typecorrigendum
dc.volumen50
sipa.codpersvinculados13517
sipa.codpersvinculados82342
sipa.codpersvinculados90468
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