Bradykinin regulates cyclooxygenase-2 in rat renal thick ascending limb cells

Simple item page
dc.contributor.authorRodriguez, JA
dc.contributor.authorVio, CP
dc.contributor.authorPedraza, PL
dc.contributor.authorMcGiff, JC
dc.contributor.authorFerreri, NR
dc.date.accessioned2025-01-21T01:07:47Z
dc.date.available2025-01-21T01:07:47Z
dc.date.issued2004
dc.description.abstractCyclooxygenase-2 (COX-2) is constitutively expressed in a subset of thick ascending limb cells in the cortex and medulla and increases when the renin-angiotensin and kallikrein-kinin systems are activated. Although the contribution of angiotensin II to the regulation of COX-2 is known, the effects of bradykinin on COX-2 expression have not been determined in this nephron segment. We evaluated expression of B2 bradykinin receptors in thick ascending limb cells containing COX-2 and the effect of bradykinin on COX-2 expression in primary cultured medullary thick ascending cells. The presence of B2 receptors was studied in renal sections by immunohistochemistry with antibodies against B2, COX-2, and Tamm-Horsfall glycoprotein. B2 receptors were detected on the apical and basolateral portion of the thick ascending cells. These cells also contained COX-2, suggesting that COX-2 expression may be regulated via B2 receptor. Incubation of cultured medullary thick ascending cells with bradykinin (10(-7) to 10(-5) mol/L) induced a significant increase on COX-2 protein expression. Maximal expression of COX-2 was observed 4 hours after exposure to bradykinin (10(-7) mol/L), effect abolished by a B2 receptor antagonist (HOE-140; 10(-6) mol/L). Prostaglandin E-2 production increased when these cells were challenged with bradykinin for 4 hours, indicating that COX-2 was enzymatically active. We have demonstrated ( 1) the presence of B2 receptors in thick ascending limb cells expressing COX-2 and ( 2) the stimulatory effect of bradykinin on COX-2 protein expression, via B2 receptors, in cultured medullary thick ascending cells. We suggest that bradykinin can affect ion transport in the thick ascending limb via a COX-2-mediated mechanism.
dc.fuente.origenWOS
dc.identifier.doi10.1161/01.HYP.0000136751.04336.e9
dc.identifier.eissn1524-4563
dc.identifier.issn0194-911X
dc.identifier.urihttps://doi.org/10.1161/01.HYP.0000136751.04336.e9
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96380
dc.identifier.wosidWOS:000222975300026
dc.issue.numero2
dc.language.isoen
dc.pagina.final235
dc.pagina.inicio230
dc.revistaHypertension
dc.rightsacceso restringido
dc.subjectcyclooxygenase
dc.subjectbradykinin
dc.subjectimmunohistochemistry
dc.subjectrats
dc.subjectkidney
dc.subjectreceptors, bradykinin
dc.subjectprostaglandins
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBradykinin regulates cyclooxygenase-2 in rat renal thick ascending limb cells
dc.typeartículo
dc.volumen44
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas