The alternative analog plasticizer BPS displays similar phenotypic and metabolomic responses to BPA in HepG2 and INS-1E cells

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dc.contributor.authorAzevedo, L. Ferreira
dc.contributor.authorMasiero, M. Miguel
dc.contributor.authorCherkaoui, S.
dc.contributor.authorCarneiro, M. F. Hornos
dc.contributor.authorBarbosa, F., Jr.
dc.contributor.authorZamboni, N.
dc.date.accessioned2025-01-20T21:04:01Z
dc.date.available2025-01-20T21:04:01Z
dc.date.issued2022
dc.description.abstractBisphenols A (BPA) and S (BPS) are endocrine-disrupting chemicals that affect energy metabolism, leading to impairment of glucose and lipid homeostasis. We aimed at identifying metabolic pathways regulated by both compounds in human liver cells and rat pancreatic beta-cells that could impair energy homeostasis regulation. We assessed the effects on growth, proliferation, and viability of hepatocarcinoma (HepG2) and insulinoma (INS-1E) cells exposed to either BPA or BPS in a full range concentration between 0.001 and 100 mu M. Both the dose and duration of exposure caused a differential response on growth and viability of both cells. Effects were more pronounced on HepG2, as these cells exhibited non-linear dose-responses following exposure to xenobiotics. For INS-1E, effect was observed only at the highest concentration. In addition, we profiled their intracellular state by untargeted metabolomics at 24, 48, and 72 h of exposure. This analysis revealed time-and dose-dependently molecular changes for HepG2 and INS-1E that were similar between BPA and BPS. Both increased levels of in-flammatory mediators, such as metabolites pertaining to linolenic and linoleic acid metabolic pathway. In summary, this study shows that BPS also disrupts molecular functions in cells that regulate energy homeostasis, displaying similar but less pronounced responses than BPA.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.fct.2022.113266
dc.identifier.eissn1873-6351
dc.identifier.issn0278-6915
dc.identifier.urihttps://doi.org/10.1016/j.fct.2022.113266
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93203
dc.identifier.wosidWOS:000830258700007
dc.language.isoen
dc.revistaFood and chemical toxicology
dc.rightsacceso restringido
dc.subjectBisphenol A
dc.subjectBisphenol S
dc.subjectHepG2
dc.subjectMechanisms of toxicity
dc.subjectMetabolomics
dc.subjectINS-1E
dc.subject.ods03 Good Health and Well-being
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleThe alternative analog plasticizer BPS displays similar phenotypic and metabolomic responses to BPA in HepG2 and INS-1E cells
dc.typeartículo
dc.volumen167
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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