Differences in expression of two endoxylanase genes (<i>xynA</i> and <i>xynB</i>) from <i>Penicillium purpurogenum</i>

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dc.contributor.authorChávez, R
dc.contributor.authorSchachter, K
dc.contributor.authorNavarro, C
dc.contributor.authorPeirano, A
dc.contributor.authorAguirre, C
dc.contributor.authorBull, P
dc.contributor.authorEyzaguirre, J
dc.date.accessioned2025-01-21T01:30:24Z
dc.date.available2025-01-21T01:30:24Z
dc.date.issued2002
dc.description.abstractA number of xylanolytic microorganisms secrete to the medium several molecular forms of endoxylanases. The physiological function of these isoforms is not clears one possibility is that they are produced under different growth conditions. To study this problem, we have used two endoxylanases (XynA and XynB) produced by the fungus Penicillium purpurogenum. These enzymes have been previously purified and characterized; they belong to family 10 and 11 of the glycosyl hydrolases, respectively. The promoters of the xynA and xynB genes have been sequenced: both present consensus sequences for the binding of the carbon catabolite repressor CreA, but otherwise show substantial differences. The xynB promoter has eight boxes in tandem for the binding of the XlnR activator and lacks the consensus sequence for the PacC pH regulator. On the other hand, the xynA promoter contains one XlnR box and three PacC consensus sequences. To investigate if these differences are reflected in gene expression, Northern blot assays were carried out. The xynA gene is transiently expressed when oat spell xylan is used as carbon source, but negligible expression was observed with birchwood xylan, xylose or xylitol. In contrast, xynB is broadly induced by all these carbon sources; this may be related to the presence of several XlnR boxes. Similar results were obtained by zymogram analysis of the expressed proteins. The different induction capabilities of birchwood and oat spelt xylan may be due to differences in their composition and structure. Expression assays carried out at different pH reflects that, despite the lack of PacC binding sites in the xynB promoter, this gene is tightly regulated by pH. The findings described here illustrate new and important differences between endoxylanases from families 10 and 11 in P. purpurogenum. They may help explain the production of multiple endoxylanase forms by this organism. (C) 2002 Published by Elsevier Science B.V.
dc.fuente.origenWOS
dc.identifier.eissn1879-0038
dc.identifier.issn0378-1119
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96738
dc.identifier.wosidWOS:000177462600017
dc.issue.numero1-2
dc.language.isoen
dc.pagina.final168
dc.pagina.inicio161
dc.revistaGene
dc.rightsacceso restringido
dc.subjectglycosyl hydrolases
dc.subjectpromoter sequences
dc.subjectgene expression
dc.subjectinverted polymerase chain reaction
dc.subject.ods07 Affordable and Clean Energy
dc.subject.ods12 Responsible Consumption and Production
dc.subject.odspa07 Energía asequible y no contaminante
dc.subject.odspa12 Producción y consumo responsable
dc.titleDifferences in expression of two endoxylanase genes (<i>xynA</i> and <i>xynB</i>) from <i>Penicillium purpurogenum</i>
dc.typeartículo
dc.volumen293
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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